Effect Of Angiotension â…¡ Type 2 Receptor Gene Polymorphism On Blood Pressure Response To CCB

Objective To explore the effect of angiotensinⅡtype 2 receptor gene polymorphism on blood pressure response to CCB and to preliminarily discuss the relationship between CCB and AT2 receptor gene polymorphism so as to provide some scientific evidence for individual therapy and healing prediction.Subjects and Methods In the research,300 new essential hypertension patients who were light and medium level were selected randomly from medicine-department clinic service,department of cordis and center of examination.Subjects with a history of cardiac or hepatic,diabetes mellitus were excluded,no symptoms of heart failure,stroke and lower-linber arterial disease. All patients have received Nifedipine Controlled Release Tablets (30mg/qd) for 8 weeks and no antihypertensive therapy for preceding two weeks,all patients'blood pressure were measured 3 times at 2,4,6,8week in the clinic service and mean value were registered as BP value. Took down their individual information (including name, sex, age, region, et al).Venous blood (5mL) were collected for measuring biochemical indicator and excluding DNA. The blood samples from the patients were collect to determine their AT2 receptor gene polymorphism by PCR combined with HRM,sequencing. The relationship among the genotypes of AT2 receptor to the therapeutic efficacy of Nifedipine was explored.Results A total of 300 qualfied patients were enro11ed in the trial.229 patients completed the final 8-week trial. A whole blood genomic DNA extraction successfully conducted and and their data were used for the polymorphism analysis. Women with 116 cases,AA,AG and GG genotypes were 31 cases (26.7%),62 cases (53.5%) and 23 cases (19.8%), A and G allele frequencies were as follows:53.4% and 46.6%. After 8 weeks, in patients with AA genotype SBP and DBP, respectively decreasing amplitude:19.84±9.89 and 12.71±7.70, AG genotype patients, respectively drop amplitude:21.00±12.42 and 14.03±9.49, GG genotype patients, respectively drop amplitude:20.04±12.05mmHg and 13.52±3.85mmHg; Antihypertensive efficiency for three genotypes (AA,AG,GG) are as follows: 71.0%,74.2%,76.0%; three genotypes difference was not statistically significant (p> 0.05). Male 113 cases, AA genotype was 61 cases (54.0%), GG genotype was 52 cases (46.0%), AA genotype and GG genotype SBP, DBP drop range, respectively: 20.20±9.25mmHg and 14.69±6.61mmHg,21.75±12.07mmHg andl3.75±7.22mmHg;Antihypertensive efficiency for two genotypes(AA,GG)are as follows:73.8% and 75.0%. In both sets of patients, blood pressure reduction and the therapeutic efficacy of the difference were not statistically significant (P> 0.05).Conclusion 1.The 1675A/G polymorphism of AT2 receptor gene was shown to no influence the response to CCB (Nifedipine Controlled Release Tablets).2.AT2 receptor gene 1675A/G allele frequencies of women:A of 53.4%, G 46.6%, male allele frequencies:A of 54.0%, G 46.0%, both male and female allele frequencies at home and abroad Similar findings are basically consistent.3. Confirmed that Nifedipine Controlled Release Tablets in patients with mild to moderate hypertension has antihypertensive effect of the exact.