Paraoxonase-1 Effect On Dichlorvos Toxicokinetics

ObjectiveIn the experiment, we studied the paraoxonase-1(PON1) effect on dichlorvos toxicokinetics to prove that it is a promising scavenger of organophosphates.Materials and Methods40 clean grade male Wistar rats were assigned to 4 groups:dichlorvos administration group (A group):dichlorvos dissolved in corn oil was injected by intraperitoneal in a dose of 10mg/kg. Paraoxonase-1 (PON1) pretreatment group (B group):PON1 was injected in the tail vein in a dose of 9600U/kg before 30min of dichlorvos administration. Treatment group(C group):atropine 0.05mg/kg and pyraloxime chloride (PAM-CI) 120mg/kg were injected via tail vein within 2min after dichlorvos administration. Co-treatment group (D group):PON1 was injected in the tail vein in a dose of 9000U/kg before 30min of 10mg/kg dichlorvos administration by intraperitoneal, atropine 0.05mg/kg and pyraloxime chloride (PAM-CI) 120mg/kg were injected via tail vein in 2 min after dichlorvos administration. Blood was collected at 3min,5min, 10min,20min,30min, 1h,2h,4h and 6h after administration from the eye veins. Plasma dichlorvos concentration was detected by liquid chromatography-mass spectra (LC-MS) method. Toxicokinetics parameters were calculated in a statistical moment model.ResultsAUC (0→∞) in group B was statistically different from that in group A and C, while it was not different from group D; there was no statistical difference between group A and group C. The statistical results of Cmax were the same as AUC (0→∞). There were no differences of MRT between four groups.ConclusionsPON1 can decrease the amount of dichlorvos that entered blood, lowed down the peak concentration, while atropine+PAM-CI does not affect metabolism of dichlorvos.