| Objective: To explore the curative effect of paraoxonase 1 in rats acute lung injury induced by dichlorvos,then comparing this therapeutic effect with the traditional atropine combined pyraloxime iodide regimen.Methods: Thirty male SD rats were randomly divided into the following 5 groups,with 6 rats in each group: control group(group A),dichlorvos group(group B),atropine and pralidoxime iodide treatment group(group C),PON1 treatment group(group D),combined treatment group(group E).Rats in groups B,C,D and E were adminstered dichlorvos by intraperitoneal injection 15 mg/kg.In group C,atropine 10 mg/kg and pralidoxime iodide 45mg/kg were injected intraperitoneally after exposure to dichlorvos.And in group D,PON1 was given caudal intravenously PON1 at a dose of 9600U/kg after poisoning.In group E,PON1 was injected caudal intravenously at a dose of 9600U/kg and was injected atropine 10mg/kg and pralidoxime iodide 45mg/kg immediately after dichlorvos administration.Rats in group A received normal saline.Lung tissue samples and blood was collected for testing at 8 hours after model establishment.Pulmonary coefficient were determined,lung tissue homogenate and blood was collected to examine the E-selectin and plasma soluble intercellular adhesion molecule concentration by ELISA method,and the changes of the lung histopathology were observed.Results: In group B,pulmonary coefficient was large,the concentration of E-selectin and s ICAM-1 increased,the lung tissue appeared neutrophil infiltration,lung edema and alveolar septal thickening.The above indicators and pathological changes of group C,D and E were less than those of group B.The indicators and pathological changes of group D and E were less than those of group C.Conclusion: PON1 is effective in the treatment of acute lung injury induced by dichlorvos in rats,and this curative effect is better than offered by atropine combined pralidoxime iodide. |
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