Expression Of RBP4 In Liver In Insulin-resistance Rats

ObjectivePreliminary study the expression of the liver retinol-binding protein 4 (RBP4) in insulin resistant (IR) rat which induced by high-fat diet.Materials and Methods30 male Wistar rats were randomly divided into basic diet group (NC) and high-fat diet group (HF), each of which has 15 rats, then normal diet and a special high-fat diet feeding were given to each group for 10 weeks respectively so as to establish insulin resistance rat model. Assessment of IR was evaluated as per glucose infusion rate (GIR) of High insulin-glucose clamp technique; measure triglyceride (TG) and total cholesterol (TC) in fasting serum, weigh epididymis and perinephrit fat, and calculate the fat/body weight ratio; the expression of RBP4 of different-group was detected by Western blot. Statistical analysis was done by SPSS13.0 software to compare the differences of each value between groups and analyze correlation of relative factors of groups.ResultsClamp results showed that: HF group of glucose infusion rate (GIR(?) 120) was significantly lower than NC group (P<0.01),there is insulin resistance in high-fat diet group (HF) In comparing with the control group (NC).Compared with the control group, the levels of high fat diet group rat liver's RBP4, TG, TC and fat/body weight ratio were significantly higher (P<0.01). Furthermore, the linear correlation analysis showed that liver fat group RBP4 expression and fat/body weight ratio was positively correlated (P<0.01), while there was no correlationship between liver fat group RBP4 expression and TG or TCConclusionThe level of RBP4 in Insulin resistance rat liver were significantly elevated, which shows positive correlation with fat/body weight ratio. Thus the level of RBP4 of insulin resistance rats may have strong correlation with insulin resistance, and the relationship between the expression of RBP4 and insulin resistance may be effected by amounts of intraabdominal fat.