HPLC Fingerprint Of Saussurea Involucrata And The Determination Of Chemical Components

In this paper, HPLC was first used to analyse different polar extracts of S.involucrata, and liquid chromatography-mass spectrometry (LC-MS) conjunction technology was used to identify the chemical components. The contents of active ingredient were determinted by plotting the standard curves. The results were as follows:The extraction technology and grade extraction including petroleum ether, ethyl acetate and n-butanol of S.involucrata was optimized, and thus more kinds of compounds were determinated.The HPLC fingerprints of different polar extracts of S.involucrata were established and 27 common peaks were assigned to bergapten, scopoletin, rutin, iso-quercitrin, honeysuckle, hispidulin through LC-MS, Syringin and chlorogenic acid were identified by comparing with the reference standards. Through the methodology study, the RSD% of the detection precision, repeatability and stability were lower than 3%, so this method was precision, stability, good repeatability, and it can be used in the quick and comprehensive quality evaluation of S.involucrata.The contents of syringin, chlorogenic acid, rutin and hispidulin were determined by plotting the standard curves. The content of chlorogenic acid and rutin were between 0.22%-2.06% and 0.27%-1.85%, which were higher than the standard of 0.15% in pharmacopoeia. This method was evaluated by linear relationship, precision, accuracy, lowest detection limit, minimum limit of quantitation and recoveries. The precision RSD were between 0.01% and 3.12%, the average recovery of each component was between 99.90% and 103.29%, which showed good detection precision and veracity.The content of two and three years seeding and tissue were also determinted and compared with 10 batches of wind S.involucrata. The solvent system used for separating flavonoids in S.involucrata was established by HSCCC. The solvent system was determined to be n-hexane-ethyl acetate-n-butanol-water (1:8:2:4), and six different chemical fractions were isolated, which include two pure chemical substances, i.e. syringin and rutin.