| Objective:Apelin-13 is a recently discovered peptide that is endogenous ligand for the G protein coupled receptor, APJ. Recent studies indicate that Apelin-13 and its receptor, APJ can regulate body temperature, influent, food intake and raising of peripheric energy expenditure in mice, thus Apelin-13 can dimin the body weight in mice. Meanwhile, ingestion and digestion has compacted connection with the gastrointestinal motility. For this reason, an attempt has been made to investigate the effects and mechanisms of Apelin-13 on gastric emptying and gastrointestinal transit after intracerebroventricular (i.c.v.) injection in mice, in this study.Methods:We studied gastric emptying by using lyophilization method and gastrointestinal transit by using charcoal meal test method. The doses of 0.3,1, 3,10(only in gastric emptying)μg/mouse were chosen to study the effects of Apelin-13 on gastrointestinal mobility. F13A,the antagonists of endogenous receptor APJ were selected as functional antagonists to study the mechanisms involved in these process.Results:1. I.C.V. injection of Apelin-13 (0.3,1,3μg/mouse) delayed gastrointestinal transit in fasted mice.2. I.C.V. injection of Apelin-13 (0.3,1,3,10μg/mouse)only 10μg/mouse can delay gastric emptying.3. I.C.V. administration of isodose F13A can significantly attenuated the delayed gastric emptying and gastrointestinal transit caused by Apelin-13.Conclusion:I.C.V. administration of Apelin-13 is able to delay gastrointestinal transit and only affect gastric emptying in high dose, moreover, all the effects are caused by the interaction of Apelin-13 and its endogenous receptor APJ.
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