| Objective:This study sought to investigate the effect of candesartan on the expression of monocyte chemoattractant protein-1, high-sensitivity C-reactive protein and so on, in order to examining the effect of candesartan on the formation of atherosclerosis.Method:There is thirty male wistar rats that were randomly divided into three groups, each group had ten:normal control group (NC),high-cholesterol control group (HC),high-cholesterol+candesartan group (HC+C),the rats were fed for eight weeks.①The concentrations of lipid in plasma were measured by chemical method at 0-week,8-week respectively, compared with the control group.②Collected the blood at 0-week and 8-week respectively, detected with enzyme-linked immunosorbent assay(ELISA), compared with each other.③The rats for 8-week feeding,anesthesia, rapid thoracotomy, aortic isolated, fixed, sliced, dehydrated, and HE staining, observed artery intima-media thickness under light microscope, and photoed.④Detected expression of MCP-1 by immunohistochemistry, calculated the average gray value, and compared.Results:①Candesartan cilexetil on the levels of lipid in atherosclerotic rats:before experiment, the lipid levels in plasma were similar among each groups, there was no significant difFerence(P>0.05);At the end of 8 weeks, concentrations of lipid in HC and HC+C group were significantly higher than that in NC group(P<0.01),there was no difference between HC and HC +C group(P>0.05).②Observed changes in hs-CRP in each group:before the experiment the levels of hs-CRP were similar among each groups, there was no significant difference(P>0.05). After 8 weeks, the concentrations of hs-CRP in HC group were significantly higher than that in HC+C group (P<0.01).③The effect of candesartan on arterial intima-media thickness:the arterial intima-media in HC group was thicker than the others, muscle fibers can be seen in the disordered membrane, inflammatory cell infiltrated outer membrane. In HC+C group than in HC group with mild intimal thickening, adventitial small infiltration of inflammatory cells.④The effect of candesartan on expression of MCP-1:MCP-1 protein expression showed a positive brown particles in the membrane and the cytoplasm, mainly seen in the neointima, also expressed in the membrane. The average gray value in NC and HC+C group(respectively, 156.94±8.158,130.98±5.941) was significantly higher than that in HC group(123.74±5.616) (P<0.01)Conclusion:①Candesartan can't reduce the lipid levels.②Candesartan can lower the expression of MCP-1 and hs-CRP, so candesartan cilexetil have anti-inflammatory effects.③Candesartan cilexetil maybe delay the process of atherosclerosis.
|
PDF Full Text Request