Expressions Of Id-1,smad4 And P21WAF1/CIP1 Protein And MRNA In Endometrioid Adenocarcinoma And Their Significance

Objective To detect the protein and mRNA expression of inhibitor of DNA binding and/or differentiation-1(Id-1), deleted in pancreatic cancer locus 4 (DPC4/Smad) and P21WAF1/CIP1 in tissues from endometrioid adenocarcinoma,endometrial hyperplasia and normal endometrium.To investigate their roles in carcinogenesis, biological behavior and to evaluate their clinicopathologic significance. Methods The expression levels of Id-1, Smad4 and P21WAF1/CIP1proteins and mRNA in endometrioid adenocarcinoma tissues(n=72),endometrial hyperplasia tissues (n=60) and normal tissues (n=30) were examined by immunohistochemistry and in situ hybridization method. Results 1. The positive rates of Id-1, Smad4 and P21WAF1/CIP1protein and mRNA in endometrioid adenocarcinoma were respectively 86.1%(62/72),44.4%(32/72),63.9%(46/72),73.6%(53/72),41.7%(30/72) and 61.1%(44/72). 2. The positive rates of Id-1 and P21WAF1/CIP1 protein and mRNA in endometrioid adenocarcinoma tissues were significantly higher than that in normal endometrium and endometrial hyperplasia(P<0.05),The positive expression of Id-1 was related to the histological grade(P<0.01), FIGO stage (P<0.01), depth of invasion(P<0.05) and lymph node metastasis(P<0.05). The positive expression of P21WAF1/CIP1 was related to the histological grade(P<0.05), FIGO stage(P<0.01) and depth of invasion (P<0.05). 3.The protein and mRNA expression of Smad4 was significantly weak in endometrioid adenocarcinoma tissues,which was negatively correlated to the the histological grade(P<0.05), FIGO stage(P<0.01) and depth of invasion (P<0.05). 4. The protein and mRNA expression of Id-1 were negatively correlated Smad4(rs =-0.652,P=0.000,rs =-0.548,P=0.000), The expression of Id-1 was not found to be correlated with P21WAF1/CIP1, The expression of smad4 was not found to be correlated with P21WAF1/CIP1. Conclusion 1. The expression of Id-1 was upregulated in endometrioid adenocarcinoma tissues, which prevented cell differentiation and promoted cell proliferation and associated with the development and progression of endometrioid adenocarcinoma. 2. The expression of Smad4 was downregulated,impling that Smad4 was inactived in process of endometrioid adenocarcinoma. 3. The expression of P21WAF1/CIP1 in endometrioid adenocarcinoma was higher than that in normal endometrium and was negatively correlated to the histological grade, FIGO stage and depth of invasion.,their expression can be regarded as latent indicatrix for evaluating biological behaviour and prognosis of endometrioid adenocarcinoma. 4. Id-1, Smad4 and P21WAF1/CIP1 gene varied in the development and progression of endometrioid adenocarcinoma. They may be a important biomarker to diagnose the early carcinogenesis and metastasis of endometrioid adenocarcinoma and to come up with direction in the targeted therapy.