| Objective: To provide several reliable parameters and indicators which can judge fertility of sperm through analysis of the relationship between the sperm morphology, rate of acrosome intactness, rate of acrosome reaction and the rate of in vitro fertilization (IVF) of ovum during the process of in vitro fertilization (IVF).Method: From July 1st, 2008 to December 30th, 2009, we chose 100 spouses to go through the IVF-ET of controlled superovulation long-term scheme in the Reproductive Medical Centre of The Third Affiliated Hospital of Guangxi Medical University. During this time, 100 cycles of data were collected for the forward-looking analysis. 100 specimens of male sperm were subject to routine analysis of sperm and detection of sperm morphology, rate of acrosome intactness and rate of acrosome reaction; we also observed and recorded numbers of collected ovum, mature ovum, fertilized ovum and unfertilized ovum to calculate the rate of fertilization. After that, statistical methods such as T test and F test of measurement data and linear correlation were applied to analyze the correlation between each parameter of the sperm and the fertilization rate.Result: The sperm specimens were divided into three groups according to the percentage of eupyrene sperm:≥5% and <10%;≥10% and <15%;≥15%. The corresponding rates of in vitro fertilization (IVF) of ovum were respectively 67.05%, 74.51% and 82.49%. Moreover, there was statistical significance in the difference of comparisons between every two groups (P<0.05). The sperm specimens were divided into two groups according to the rate of acrosome intactness:≤85% and >85%. The corresponding rates of in vitro fertilization (IVF) of ovum were respectively 75.57% and 89.19%. Moreover, there was statistical significance in the difference of comparison between the two groups (P<0.05). The sperm specimens were divided into three groups according to the rate of acrosome reaction: <14%;≥14% and≤25%; >25%. The corresponding rates of in vitro fertilization (IVF) of ovum were respectively 73.45%, 77.19% and 82.74%. Moreover, there was statistical significance in the difference among every group (P<0.05). The sperm specimens were divided into two groups according to the percentage of normal sperm morphology: <15%; >15%. The corresponding rates of acrosome intactness were respectively 79.51% and 87.27%. Moreover, there was statistical significance in the difference of comparison between the two groups (P<0.05). In the multi-linear analysis, the correlation coefficients of sperm morphology, rate of acrosome intactness and rate of acrosome reaction were respectively0.352,0.497,0.271,0.045,0.005,0.017, All these difference had shown statistical significance. The normalizing coefficient of Rate of Acrosome Intactness is the biggest.Conclusion: For the men whose routine semen analysis is normal, about 50% of them have abnormal semen shape at different extent. The fertilization rate of ovum improves with the increase of the percentage of semen in normal morphology; if the morphology rate of normal semen is more than or equal to 5%, a relatively better in vitro fertilization rate will be gained. Thus, it is possible to carry out normal in vitro impregnation helping pregnancy. For the men with normal routine semen analysis, about 65% of them have an intact acrosome rate of sperm more than 85%. With the increase of the same rate, the fertilization rate of ovum obviously is improved; some 25% men have the same rate less than 85%, but they still have relatively better in vitro fertilization rate. For the men whose routine semen analysis is normal, 78% of them have an acrosome reacted rate of sperm more than or equal to 14% (among which 53% of them have a rate more than 25%). In the in vitro fertilization, the fertilization rate is increased with the increase of the percentage of acrosome reacted rate of sperm; but the men have the same rate less than 14% (22% of the above men) still have relatively better in vitro fertilization rate. If the patients whose acrosome reacted rate less than or equal to 14% have not ideal normal morphology rate of semen and the intact acrosome rate of sperm, it can be considered to directly carry out 50%IVF-50%ICSI or ICSI, which will save operation fee and treatment time for the patients. The above three rates are all important elements influencing the fertilization of ovum. In vitro fertilization rate of ovum has relatively strong relation with the above indexes; among which intact acrosome rate of sperm has the maximum influence upon IVF rate. For the men having normal routine semen analysis, if their normal morphology rate of semen is more than or equal to 5%, the test of intact acrosome rate of sperm and acrosome reacted rate of sperm need not to deal with routine check items before in vitro fertilization.
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