| Background: With the development of molecular biology and tumor reveals the factors of cure,Cancer gene and its treatment of molecular biology technology has become the hotspot.The medical research shows that, before,All tumors and development are due to some cancer genes related to change,One tumor-suppressor gene inactivation or missing in the tumor and plays an important role in development.Since Bednarek etc. In 2000 DiaoQiang method using cloning identification with a similar FHIT gene new tumor-suppressor genes WWOX WWOX since, as a kind of new, is located in chromosome tumor-suppressor genes, caused the people more and more high attention.The existing research shows that WWOX genes as normal sexual brittleness sites on the gene, vulnerable to external environment factors. These environmental carcinogenic factor can make WWOX gene locus of brittle, and in early may, can cause for heterozygous and lack of pure lost one or a few explicit, and the variation of the WWOX or cause WWOX reduction, even without expression. Now also reported by deflection caused by splicing variants, thus affecting the WWOX apoptosis. Cancer genes promotes normal cells undergo malignant transformation, tumor formation has two main mechanism: one is overly activation cell cycle, in order to make a cell increment of restrictive split. On the other hand is programmed cell death, restrain to cell death. Tendentiousness Cell proliferation, speed, and not the mutated cell apoptosis mechanisms through comprehensive balance, the result is the main mechanism of tumor formation. WWOX promote apoptosis, tumor-suppressor so tumor-suppressor genes WWOX disorders or variation may cause tumors.The origin and development of the tumor is regarded as one of the many steps in multiple genes complicated process. In the complex process, anti-oncogene inactivation or lack of common and important is caused cancer molecular biology. It is possible to resist cancer important protection mechanism.The most common oral squamous cell carcinoma is one of the high incidence, its mouth, low survival, serious harm to human health. Oral squamous cell carcinoma of the occurrence and development of cancer genes involved and tumor-suppressor genes, the structure and function of the experimental research, WWOX in oral mucosal pre-cancerous and oral expression of squamous cell carcinoma.Objective: to detect the expression of WWOX in OSCC and to explore its role in the development of OSCC and its future prospects. Methods: From August 2008 to November 2009, we obtained 41samples from the stomatology hospital of Jilin university , including 6 cases of normal oral mucous,5 cases of hyperplasia epithelia,12 cases of dysplasis epithelia and 18 cases of oral squamous cell carcinoma (OSCC).We detected the expression of WWOX mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR), respectively.Results:We detected the expression of WWOX mRNA in all oral mucous tissue and epithelia hyperplasia tissue . We detected the expression of WWOX mRNA in 10 epithelia dysplasis tissue and 12 OSCC . There were significant difference between normal oral mucous group, epithelia hyperplasia group and epithelia dysplasis group , OSCC .Except that there were no significant difference in ing1 mRNA expression between oral mucous group and epithelia hyperplasia group (P>0.01),there were significant difference between any two group. Conclusions:1.WWOXmRNA oral mucosal tissue in the normal and abnormal growths simple organization, organization and oral expression level of squamous cell carcinoma in a progressive decline.2.WWOXmRNA in oral expression of squamous cell carcinoma with normal oral mucosa and simple organization is decreased obviously. 3.WWOX tumor-suppressor genes on the research will help reveal oral squamous cell carcinoma occurs in the process of development, and guide the molecular mechanism of tumor genetic diagnosis, treatment and prognosis. |
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