The Experimental Study Of Weitongxiaopi Decoction Influence On Gastrointestinal Motility In Functional Dyspepsia Rats With Liver Depression

ObjectiveThis experiment devised model of functional dyspepsia in liver depression rats which were angered by clipping tails. To investigate the effect of weitongxiaopi decoction (WTXPD) to gastrointestinal motility disorder by contrasting the effect between different doses of WTXPD and domperidone.Methods1. Dvision of animal models60 rats were randomly divided into 6 groups, the normal group, model group, domperidone group, low dose group, middle dose group, and high dose group. In addition to the normal group, other groups were devised model of functional dyspepsia in liver depression rats which were angered by clipping tails. To clip the distal 1/3 rat tails with tongs for 30 minutes, and 4 times one day for 7 days.2. Intragastric administrationRats from homologous groups were feed with 2ml different doses of WTXPD or domperidone twice per day for 14 days. Rats from normal and model group were given equal volume of normal saline.3. Measure rats plasma MTL level, serum NO level, small intestinal propulsive ratioAfter the last administration, rats were given ink semi-solid paste. Waiting 30 minutes, then to get blood from the abdominal aorta, and measure the total length of the small intestine and the distance from the pylorus to the ink front. To measure the MTL level and NO level by radioimmunoassay, and to calculate the small intestinal propulsive ratio according to the formula. 4. Production of gastric tissue sliceTo take full layer tissue of middle gastric wall about 0.5cm×0.5cm, which were fixed in 4% paraformaldehyde.24h later, embedded in paraffin, sliced thickness of 4pm, hematoxylin-eosin (HE) staining, then observing the tissue change under the light microscope.Results1. During clipping tails, fighting to each other among rats rising gradually, also food intake reduction, restlessness, irritability, loose stools thin, foul fecal smell, withered coat, gloss reduction, slow response changes. After treatment, these symptoms improved obviously.2. In model group, plasma MTL level (79.81±39.10pg/mL) was significantly lower(normal group 116.49±19.38pg/mL, P<0.01), serum NO level (61.34±21.11umol/L) was significantly higher (normal group 36.23±11.75umol/L P<0.01), and small intestinal propulsive ratio (45.27±8.78%) was significantly lower (normal group 53.74±6.76%, P<0.01). After treatment, plasma MTL level (180.94±31.51pg/mL,135.84±38.87pg/mL,170.05±36.33pg/mL, 178.18±78.40pg/mL) were significantly higher (P<0.01), serum NO level (24.68±8.18umol/L,43.96±25.25umol/L,32.31±15.93umol/L,29.45±16.57umol/L) were significantly lower (P<0.01), and small intestinal propulsive ratio (65.12±7.07%, 59.36±7.72%,64.02±7.58%,64.44±7.82%)were significantly higher (P<0.01) in every treatment group. For every index in WTXPD groups, middle dose group had significant difference (P<0.01 or P<0.05) compare with low dose group, but had no significant difference compare with high dose group (P> 0.05).3. Gastric tissue in treatment groups, model group and normal group had no significant difference.Conclusion1. The WTXPD could obviously improve the functional dyspepsia symptoms in liver depression rats.2. The WTXPD could significantly improve gastrointestinal motility disorder of functional dyspepsia in liver depression rats, and significantly advance plasma MTL level, reduce serum NO level, and accelerate small intestinal propulsive ratio.3. The effect of middle dose group WTXPD was best.