Expression Of Musashi-1 And P63 In Human Endometrium And Their Significance

Objective In our study, we examined P63 and Musashi-1 protein expression in fetal endometrium, reproductive endometrioid epithelia,endometrial hyperplasia, endometrioid carcinoma tissue, and then study the role of P63 and Musashi-1 in physiologic and pathological proliferating endometrium, in saerch of possible endometrium stem cell markers.Methods P63 and Musashi-1 were detected by immunohistochemical SP staining in twenty cases of fetal endometrium from 12 to 30 weeks, twenty cases of reproductive normal endometrium including proliferative and secretive phase, forty cases of endometrial hyperplasia and fifty cases of endometrioid carcinoma. Select some specific samples for double-immunohistochemical SP-SAP staining of P63/Musashi-1, P63/ER-a and Musashi-1/ER-a. Make statistical analysis with 13.0 SPSS statistical software.Result (1)P63 expression:In fetal endometrium, P63 protein was identified in fetal endometrioid epithelia cells, concentrating on the superficial epithelium, the level of P63 expression decreased with the increase of the embryonic age. In reproductive endometrium, there was only one proliferative case showing P63 protein positive. Positive cells scattered in the basal of glandular epithelium. The expression of P63 in endometrioid carcinoma and endometrial hyperplasia were higher than normal endometrium(P<0.05).In endometrioid carcinoma, positive cells were identified in both cancer nests and stroma, especially where squamous differentiation,as while as in epithelium. In endometrial hyperplasia,the numember and staining of some cases were higher than in endometrioid carcinoma. While there was no significant difference between endometrioid carcinoma and endometrial hyperplasia (P>0.05). (2)Musashi-1 expression:In fetal endometrium, the level of Musashi-1 expression decreased with the increase of the embryonic age. Positive cells were identified in both epithelium and stroma. In reproductive endometrium, there were only two proliferative and one secretory cases showing Musashi-1 protein positive. The expression of Musashi-1 in endometrioid carcinoma and endometrial hyperplasia were higher than that in normal endometrium(P<0.05), while there was no significant difference between the endometrioid carcinoma and endometrial hyperplasia(P>0.05). Positive cells were identified in both epithelium and stroma. (3)The result of immunohistochemical double-staining:we detected P63/Musashi-1 double positive cells in endometrioid carcinoma and endometrial hyperplasia. The everge percentages of positive cell were 1.04%±0.21% and 3.12%±0.34% respectively,which numbers were lower than that of single-positive cell in the same sample(p<0.05). We detected P63/ER-a and Musashi-1/ER-a double positive cells in endometrial hyperplasia, mainly in stroma.Conclusions (1)P63 protein and Musashi-1 protein are involved in the growth of fetal endometrium, cyclical regeneration of reproductive endometrium, as well as proliferation of endometrioid carcinoma and endometrial hyperplasia. The localization of these cells coincide with the predicted endometrium stem cell. (2)There were some endometrium cells co-expressing P63 and Musashi-1,and the number approaches to that of the stem cells, indicating that double-staining of P63 and Musashi-1 would contribute to identifying the endometrium stem cells. (3)There are some endometrium cells co-expressing P63/ER-a, Msi-1/ER-a, whose localization was related to the niche of endometrium stem cell, indicating that strogen acts on endometium stem cells by the niche.