| Objective:To investigate the clinicipathological significance of Ezrin Protein overexpression and DNA ploidy detection in uterine cervical cancers.Materials and Methods:Total 130 cases of paraffin embedded cervical lesion blocks, including 15 cases of normal cervical epithelium,51 cases of cervical intraepithelial neoplasia (CIN),6 cases of cervical glandular intraepithelial neoplasia (CGIN),53 cases of squamous cell carcinoma (SCC) and 5 cases of adenocarcinoma were selected from Department of Pathology, The First Affiliated Maternity and Infant Hospital of Tongji University, Jilin Railway Hospital, and Yanbian Maternity and Infant Hospital in the period of 2000-2009. The FIGO (Federation Internationale de Gynecologie et d'Obstetrique) stages of SCC were as follows:11 cases of stage I, 14 cases of stageâ…¡,19 of stageâ…¢,9 of stage IV; FIGO stages of cervical adenocarcinomawere as follows:1 cases of stageâ… ,2 cases of stage II, and 2 cases of stageâ…¢. In which,28 cases of cervical cancers showed lymph node metastasis, and 25 cases of them showed non-metastasis; twenty-four cases of SCC were early stage (I-IIA), and twenty-nine cases of SCC were late stage (IIB-IV). Ezrin protein expression status was detected in normal cervical epithelium, CIN, CGIN, SCC, and adenocarcinoma by immunohistochemistry. And DNA ploidy status of cervical lesions was analyzed by flow cytometry.Results:Ezrin protein was negative in most of normal cervical squamous and glandular epithelia. Several positive cases mainly showed that Ezrin protein located in the membrane and cytoplasm adjacent to the inside of cervical gland, and the positive cells in normal cervical squamous epithelia were mainly located in the basal layer of epithelium. However, Ezrin protein showed nuclear or cytoplasmic staining pattern in atypical and cancer cells of uterine cervix. The positive rates of Ezrin protein expression were significantly increased in CIN (86.3%,44/51), CGIN (100%,6/6), SCC (88.7%,47/53), and adenocarcinoma (100%,5/5) than it in normal cervical epithelium (13.3%,2/15) (P<0.01). The strongly positive rates of Ezrin protein were 65.2% in keratinizing SCC (15/23) and 80.0% in non-keratinizing SCC (24/30) (P>0.05),70.8% inâ… -â…¡A (early stage) (17/24) and 75.9% inâ…¡B-â…£(late stage) (22/29) (P>0.05),96.4%in SCC with lymph node metastasis (27/28) and 48.0% in SCC with non-metastatic cases (12/25) (P<0.05). Additionally, DNA ploidy detection showed that all 15 cases of chronic cervicitis and 21 of CIN-â… and CIN-â…¡were DNA diploidy, while the detection rates of DNA aneuploidy were 18.8% in CIN-â…¢(3/16),16.7%in CGIN (1/6),49.1% in invasive SCC (26/53) and 80.0% in invasive adenocarcinoma (4/5), the difference was significant comparing to the normal cervical epithelium and CIN-I-II lesions (P<0.01).Conclusions:â‘ The expression pattern of Ezrin protein would be helpful for predicting the tendency of atypical and/or malignant changes from normal cervical epithelium.â‘¡Ezrin protein could be used as early diagnosis of cervical cancers and their precancerous lesions.â‘¢The detection of Ezrin protein expression is valuable to predict the status of lymph node metastasis, and it could be used as the prognostic marker for uterine cervical cancers while Ezrin protein expression was detected combined with the DNA ploidy analysis. |
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