| Objective:We observed systematically curative effect of preventing and treating Diabetic nephropathy (DN) rats by Shenbaining through clinical and experiment researches.We inquired into mechanism of traditional Chinese medicine(TCM) on DN and mechanism of Shenbaining on Preventing and treating DN.Methods:In the study,we use the left kidney of the STZ-induced DN rat.The TCM group will dissolve shenbaining in saline,prepared into suspension and it was given 2.97g/kg,ltime/day,orally,2ml/time;The positive control group lotensin was prepared into a suspension,and also was given 0.9mg/kg,2ml/time,ltime/day,orally.Control group and the sham group to the same volume of saline solution 2ml,ltime/day. Then we observed the condition of their urine protein excretion,mice kidney weight and body weight changes.Using immunohistochemistry to qualitative and quantitative detection of kidney AGEs.Results:12 weeks later, it can be found in the control group that glomerular atrophy ball,balloon chamber slightly increased the glomerular afferent artery with slight endothelial cell hyperplasia and fibrosis,basement membrane thickening slightly,distal convoluted tubule expansion,renal interstitial congestion,edema,blood cell infiltration and mild interstitial fibrosis. Compared with the positive control group,the CTM group's body weight increase was significantly better,the kidney weight index were decreased,but the difference was not significant.At the 12th week,the TCM group urine albumin,NAG, ACR was significant different from the control group and the sham group;AGEs condition of the TCM group were significantly lower with,there were significant differences.Conclusion:Shenbaining can increase the DN rats'weight, improve the kidney weight/body weight ratio,significantly reduced renal AGEs content levels,increased the expression of glomerular and tubular structure to protect and improve renal function. |
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