Preliminary Study On Chemical Constituents And Bioactivity Of Extractions From Capparis Spinosa L. Stem

Capparis spinosa L. was investigated as research subject in this paper. The main volitile chemical contents, allelopathy effect of stem-water extraction, extraction process of total flavonoids and antioxidation activity in vitro and chemical components of volatimatter have been analysed in this paper. At the same time, a monomer from ethanol extraction of Capparis spinosa L. stem was isolated, purified and structurally identified. The results were described as follows:1. Uniform design was used to optimize the extraction process of flavonolds from Capparis spinosa L. stem. The results showed that ultrasonic-microwave co-assisted extraction was the best method compared with merceration, ultrasonic-assisted extraction and microwave-assisted extraction. The optimum extraction conditions: extraction time 45 min; the ratio of material to liquor 45:1; the concentration of ethanol 90% and the microwave power 311W.2. The experiments of antioxidation in vitro of flavonolds showed that it had strong anti-lipid oxidation capability and reduction power, strong clearance of superoxide anion, DPPH and hydroxyl radical, and certain chelating ability.3. Aqueous extraction of Capparis spinosa L. stem had a strong ability of allelopathy against the seed of radish, Chinese cabbage and ipomea.4. Steam distillation was applied to extract volatimatter from Capparis spinosa L. stem. GC-MS was applied to identify 12 kinds of essential components.5. Separation and purification of ethanol from Capparis spinosa L. stem was performed using TLC and column chromatography, by doing which a kind of white crystal was obtained. The structure of the compound was identified asβ- sitosterol through MS, 1HNMR and 13CNMR.