The Effect Of Sevoflurane, Isoflurane, Enflurane In General Anesthesia Of Liver Cancer Patients And Induced Apoptosis Of HepG2 Cells Cultured In Vitro

PARTⅠSevoflurane, isoflurane and enflurane-induced apoptosis of HepG2 cells cultured in vitroObjective To study the effect of sevoflurane, isoflurane and enflurane in inducing apoptosis of HepG2 cells cultured in vitro.Methods The effect of sevoflurane, isoflurane and enflurane on the apoptosis of HepG2 cells was observed using PI staining and flow cytometry.Results The apoptosis level are (0.46±0.21)%,(0.52±0.10)%,(1.15±0.32)%(sevoflurane), (0.63±0.09)%,(0.69±0.13)%,(1.39±0.47)% (isoflurane), ( 0.51±0.07 ) %,( 0.42±0.11 ) %,( 0.62±0.29 ) % (enflurane),respectively,after treated with different concentration for 24h, while the level in control group was (0.37±0.17)%. 24h after sevoflurane,isoflurane and enflurane at concentration of 10 and 20MAC was applied to HepG2 cells,there are no significant difference compared with that in the control group;24h after sevoflurane, isoflurane and enflurane at concentration of 30MAC was applied to HepG2 cells,the percentage of apoptotic cells increased in sevoflurane and isoflurane treated groups.Conclusion Isoflurane and enflurane in superclinical dosage could induce apoptosis of HepG2 cells .And there are no effect of enflurane on HepG2 cells cultured in vitro. Objective To observe sevoflurane, isoflurane and enflurane treated with liver carcinoma hepatectomy patients.Method Eighty ASAⅠorⅡpatients undergoing elective liver carcinoma hepatectomy under general anesthesia were randomly allocated to one of four groups with 20 patients in each group: group S1 ,gave sevoflurane inhalation anesthesia.;group S2 ,isoflurane;group S3, enflurane;group P,gave intravenous anesthesia of propofol. Induction was began after building up an intravenous transfusion,HR,BP,RR were monitored.Anesthetic agents were given intravenous by turns,Midazolam0.1mg/kg,propofol 1.5mg/kg ,vecuronium 0.1mg/kg,fentanyl 3μg/kg,tracheal intubation was performed in 3 minutes after the injection.After intubation,anesthesia was maintained with sevoflurane, isoflurane ,enflurane in groupp S and propofol in group P .Graded intravenous injection of vecuronium to maintain muscle relaxation,adjusted the propofol concentration according to hemodynamics.The occurrence of choking was observed and recorded . Moreover the following variables were recorded:.MAP,HR before induction,after intubation,1,3,5,1 0 minutes after intubation,at incision,30,60,1 20 minutes after incision,at the end of surgery,at palinesthesia,before extubation,and 1,5,1 0,20,30 minutes after extubation.Vasoactive drugs,time for return of spontaneous ventilation,return of analepsia , extubation and postoperative complications were also recorded.The concerntration of ALT,AST,TBIL,DBIL,TP and ALB were examined at preanesthesia(T1),the first day post-operation(T2) , the fifth day post-operation(T3), the tenth day post-operation(T4) and the twentieth day post-operation(T5)Results No differences were observed in four groups after sevoflurane, isoflurane and enflurane anesthesia compared with patients after propofol anesthesia in vital signs and postoperative liver function.Conclusion The effect of sevoflurane, isoflurane and enflurane on anesthesia for liver carcinoma hepatectomy is satisfactory...