| Objective:The present experiment is to explore the relationship between NF-κB, Notch-1, Ki-67 and colon cancer development through investigating the expression of NF-kB, Notch-1 and Ki-67 in colonic mucosa in patients with colon cancer, for the clinical diagnosis and usefull prognostic indicator. Methods:The 110 cases of paraffin block from the department of pathology of the affiliated hospital of Luzhou medical college from December 2008 to January 2010,20 colon adenoma,90 colon cancer, with 45 well-differentiated,33 moderately differentiated and 12 poorly differentiated, were assigned as the experimental group. The 10 cases of normal colon,5cm away from carcinoma tissue, were included in control group. Histological type of the specimen was confirmed by pathological diagnosis. NF-κB, Notch-1, Ki-67 expression was assessed by immunohistochemistry method. Using SPSS 17.0 for statistical analysis, count data was analyzed by chi-square test and Fisher exact probabilities test, non-parametric statistics used rank sum test and correlation analysis was made by Spearman rank correlation analysis. Results:(1) We demonstrated that the positive rate of NF-κB in the sequence of normal colon mucosa-adenoma-carcinoma was 10%,35%, 56.7%. The positive rate of NF-κB in colon cancer was higher than that of normal mucosa group (X2=7.853,p<0.05), and compared with colon adenoma group, had no difference (X2=3.082, p=0.079). The positive rate of Notch-1 in the sequence of normal colon mucosa-adenoma-carcinoma was 10%,35%,56.7%. The positive rate of Notch-1 in colon cancer was higher than that of normal mucosa group (X2=6.586, p<0.05), and compared with colon adenoma group, had no difference (X2=0.358,p=0.549). The positive rate of Ki-67 in the sequence of normal colon mucosa-adenoma-carcinoma was 24.4%, 47.22%,75.6%. The positive rate of Ki-67 in colon cancer group was higher than that of colon adenoma group and normal colon mucosa group (X2=7.275,13.228,p<0.05). (2) We founded that the positive rate of NF-κB in colon cancer concerned with tumor size, different depth of invasion, lymph node metastasis and extent of differentiation (p<0.05), had no significant relationship with age, sexuality and histology category (p>0.05). The positive rate of Notch-1 in colon cancer concerned with tumor size and extent of differentiation (p<0.05), had no significant relationship with sexuality, age and lymph node metastasis (p>0.05). The positive rate of Ki-67 in colon cancer concerned with the differentiation degree and lymph node metastasis (p<0.05), had no significant relationship with other clinical pathological factors (p>0.05). The expression of NF-κB, Notch-1 and Ki-67 by rank sum test was distinguished at distinct differentiation degree in colon cancer. The lower differentiation, the higher positive rate was. The difference was statistically significant(X2=7.2,9.846,20.489, p<0.05). (3) We also founded that the expression of NF-κB and Notch-1 in colon cancer had positive correlation (r=0.831, p<0.01), the expression of Notch-1 and Ki-67 in colon cancer had positive correlation (r=0.986, p<0.01), the expression of NF-κB and Ki-67 in colon cancer also had positive correlation (r=0.861,p<0.01). Conclusions:All above, our data imply that (1) the expression of NF-kB in the sequence of normal colon mucosa-adenoma-carcinoma was gradually increased. The expression of NF-κB in colon cancer was higher than that of normal colon mucosa group. The positive rate of NF-κB in colon cancer is associated with tumor size, stage, degree of differentiation and lymph node metastasis. Detect the expression of NF-κB in colon cancer is a biological indicator as early diagnosis, distinguish invasion and prognostic indicator. Inhibit NF-κB specifically can inhibit the development of colon cancer, improve the prognosis. (2) the expression of Notch-1 in the sequence of normal colon mucosa-adenoma-carcinoma was gradually increased. The expression of Notch-1 in colon cancer was higher than that of normal colon mucosa group. The positive rate of Notch-1 in colon cancer is associated with tumor size and degree of differentiation. The expression of Notch-1 may play an important role in the development of colon cancer. (3) the expression of Ki-67 in the sequence of normal colon mucosa-adenoma-carcinoma was gradually increased. The expression of Ki-67 in colon cancer group was higher than that of colon adenoma group and normal colon mucosa group. The positive rate of Ki-67 in colon cancer concerned with the differentiation degree and lymph node metastasis. The lower degree of differentiation, as well as lymph node metastasis, the higher expression of Ki-67 may have. Ki-67 had comparatively significance at early diagnosis in colon cancer. It's waiting for further studying of confirmation to forecast the infiltration and recrudescence of colon cancer. (4) the analysis of correlation about expression among NF-κB, Notch-land Ki-67 showed that, the positive rate of Ki-67 reliably reflects the proliferation activity of colon cancer cells. Positive correlation of NF-κB, Notch-1 and Ki-67 in colon cancer shows that, the high expression of NF-κB and Notch-1 in colon mucosa is related with the pathogenesis and development of colon cancer. NF-κB and Notch-1 in colon cancer had positive correlation, which shows that NF-κB signaling pathway and Notch signaling pathway may interact together in the pathogenesis and development of colon cancer. |
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