Prokaryotic Expression Of Human Annexin A3 And It's Expression Level In Lymphocytes Of Children With Asthma

Pediatric asthma is a common chronic respiratory disease of children. In recent years, its incidence in the world has been raised, and the prevalence of asthma in children was up to 10% or more in developed countries. Because asthma is often recurrent and difficult to cure, so it seriously not only affects the health of children, but also brings heavy financial burden and mental stress to the parents of children. Our laboratory have been reported with membrane lymphocytes in asthmatic mouse A3 protein was unregulated, suggesting that membrane protein A3 may play an important role in the formation of asthma. This study reports that gene anxA3 was cloned by the primers which were design as the sequences of anxA3 published on NCBI. The sequence we obtained was agreement with the reports online. The ORF of anxA3 is 1026 bp, encoding 341 amino acids. We use the prokaryotic expression vector pEASY to constructed recombinant vector pEASY-anx A3 which expressed in E. coli BL21 (DE3) pLysS. Nickel affinity chromatography was used to purify protein ANX A3. Using commercial antibodies and detecting by Western Blot Identification, the protein which was expressed and purified was ANX A3. We use the purified protein ANX A3 to immune rabbit. The serum was collected and antibodies were extracted and purified. Point mark was used to test the purified antibody whether was protein ANX A3. The expression protein ANX A3 was identified by western Blot using normal children and children with asthma. The results showed that the expression of protein ANX A3 was increased in children with asthma, indicating that protein ANX A3 may be related to asthma in children. In the future, ANX A3 may become an important indicator of prognosis of clinical judgments in occurrence of asthma.