| Research background and objective:Male infertility is the most common and refractory male urology disease. With the pollution of the environment getting worse, the influence of drug abusing and the accelerated pace of life, the rates of male infertility is increasing year by year. The causes of male infertility is complicated, but Oligozoospermia and asthenozoospermia is one of the important reasons. However, nearly half a century, the progress of male infertility research is slow, and the mechanism of male infertility is not very clear. Therefore, there is not an effective drug in the treatment of male infertility.At present,drugs in clinical application are mostly improving the quality of sperm through improving the antioxidation ability, but the absence of suitable remedies, effect and repeatability on male infertility treatment. It is summed up a set of dialectical disease differentiation, treating diseases based on different patients and causes of the traditional chinese medicine theory in long-term clinical practice, in particular, to treat Oligozoospermia and Asthenozoospermia by activating blood and dissolving stasis associated with warming kidney-yang and nourishing kidney shade. Therefore it is imminent to study a new method of male infertility treatment. In recent years, it is an important breakthrough to treat Oligozoospermia and Asthenozoospermia by the effective Monomer composition of Chinese medicine in animal studies. In this experiment, we study the effect of Compound Chinese Herbal Monomer Recipe which were made of resveratrol,lycium barbarum polysaccharide(LBP),quercetin and L-camitine on spermatogenic disturbance in mice induced by 60Co-γ, and explore the best effective dose.Methods:After the seventy-two male Hunming mice adaptive feeding two weeks, they were randomly divided into 5 groups completely:group A of 12 rats, group B, C, D, E of 15 each. In addition to group A, other groups were established models of spermatogenic disturbance by the irradiation of the 6 Co-γwith 6 Gy.After feeding one week and the general condition of the mice in each group stabilitated, group C, D and E group were fed different concentrations Compound Chinese Herbal Monomer Recipe with 0.5% sodium carboxymethyl cellulose prepared:low-dose (40 mg/kg·d-1), middle dose (80 mg/kg·d-1), high dose (160 mg/kg·d-1) solution, and The normal group and the control group were treated 0.5% of sodium carboxymethyl cellulose saline. once a day, administered orally 35 days. The general condition,physical sign and body weight of mice were recorded, and 24 hours after finishing gavage, the weight of testes were measured and the testicular organ index were calculated; Then taking blood serum by picking eyeball, separating serum, the levels of FSH, LH, T and E2 determined by enzyme-linked immunosorbent assay and the ratio of T/E2 were calculated; To take bilateral testes, histological changes of testis were observed on the left testis by HE staining, and the expression of c-kit were observed on the right testis by immunohistochemical staining.Results:At the end of the experiment, there are six-five mice survived finally. Two mice of each group in group B, Cand E and one mouse of group D were dead. In group A, the general condition of mice is good, body hair smooth, body weight increased gradually; after two days by by the irradiation of the 60Co-y with 6 Gy, mice of groupB, C, D and E is lack of energy, limb weakness, the activities of retardation, crouching and moving less,loss of appetite, dark color and a plush body hair removal phenomenon, skin relaxation, and body weight beganning to decline and so on. After group C, D and E were fed on different concentrations of Compound Chinese Herbal Monomer Recipe, the above-mentioned signs were all varying degrees improvement, while the mice of group B was not obvious signs of improvement. Compared with those of Group B, the body weigh,the testis weight and the testis index of Group D(80 mg/kg·d-1)were improved obviously(P＜0.05)and closed to those of Group A(P＞0.05). those of Group C and E were improved, but were still lower than those of Group A(P＜0.05). The levels of the serum reproductive hormone of Group C,D and E had some degree of recovery, but those of Group D were close to normal(P＞0.05). The layers of spermatogenic cells, the number of sperm and the positive expression of c-kit in spermatogenic tubules of Group C,D and E were superior to those of Group B, especially in Group D(80 mg/kg·d-1).Conclusion:Compound Chinese Herbal Monomer Recipe could promote effectively the recovery of spermatogenic disturbance in mice induced by 60Co-γ, and the best dose was 80 mg/(kg·d). |
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