Experimental Study On L-carnitine Plus QianLieShuTong In Treating Immunological Infertility Of Male Mice

Objective:Production of animal models of male immune infertility. Taking different drugs by measuring the immune infertile males before and after treatment of serum AsAb in the value of OD changes and changes in sperm parameters was observed L-carnitine combined QianLieShuTong to the immune infertility treatment of male mice, and explore AsAb on sperm parameters.Material and Methods:With the same kinds of sperm and Freund's adjuvant immunization Kunming male mice, ELISA method validation to obtain animal models of immune infertility, while the establishment of the normal control group; were given L-carnitine plus QianLieShuTong, L-carnitine, QianLieShuTong, Prednisone, normal saline, by gavage, in one month; Weili Automatic Computer sperm analysis to measure the sperm density, linear velocity and live rates, and statistical analysis.Results:After the animals were immune serum AsAb male model group were all positive, no artificial immune control group were negative. Determination of OD values compared:combined treatment group, L-carnitine group, Qianlieshutong group, prednisone group OD values were lower than the saline group, the difference was statistically significant (P<0.05); L-carnitine group OD value higher than the front passage on group and prednisone group, but no statistical significance (P>0.05); combined treatment group although the OD values lower than the L-carnitine group, through group and prednisone Qianlieshutong group, but can not think that the difference was statistically significant (P>0.05). Determination of sperm parameters showed: L-carnitine group and the combined treatment group Qianlieshutong the parameters of the sperm is superior to L-carnitine group, Qianlieshutong group, prednisone group, the difference was statistically significant (P<0.05), and compared with the control group no significant difference (P>0.05); animal model group sperm parameters of four treatment groups compared with the saline group had different degrees of improvement was statistically significant (P<0.05); the saline group of three sperm parameters were lower than the control group, the difference was statistically significant (P＜0.05).Conclusions:With the same kinds of sperm and Freund's adjuvant immunized animals can be successfully produce anti-sperm antibody-mediated immunity is not sterile animal model; AsAb the various parameters can be reduced by affecting sperm fertility of male mice; L-carnitine combined QianLieShuTong to clear the treatment of immune infertility male mice there is a genuine effect.