The Experimental Study On The Effect Of Dahuang Zhechong Pill On Atherosclerotic Renal Artery Stenosis-Caused Renal Ischaemia Model In Rabbits

Objective To observe the effects of Dahuang Zhechong Pill on improvement of renal atherosclerosis, pathological changes and inflammatory factor in rabbit. Methods Thirty five healthy New-Zealand white rabbits were randomly divided into groups control, model, high-dose, middle-dose of Dahuang Zhechong Pill (DZP) and blank. Before the silver clip tightening surgery of establish left kidney artery ligation models, the rabbits in model group, TCM group(treated by DZP) and control group(treated by Simvastation) were feed with ordinary food for one week. After the operation, all rabbits were feed with high fatty food for 12 weeks. At the same time, the TCM groups given Dahuang Zhechong Pills in high, medium doses, respectively by intragastric administration.The control group were given Simvastation. The model and the blank groups were given equal amount of distilled water. Aftre 12 weeks, the aorta, renal artery and renal pathological morphology were observed by image analysis system (Motic Images Advanced 3.0). The thickness and area of tunica intima and tunica media were measured. In addition, the sections were analyzed for renal artery stenosis.The levels of serum cholesterol (TC), triacylglycerol (TG), low density lipoprotein(LDL),high density lipoprotein (HDL), renal function were measured. ELISA was used to measure the contents of TNF-a,IL-1β. Results 1. Atherosclerotic renal artery stenosis-caused renal ischaemia models were established successfully in rabbit. 2. Compared with the model group, the renal artery stenosis and thickness of renal artery tunica media in treated groups were decreased significantly (P<0.01). At the same time, the degrees of renal interstitial fibrosis were decreased (P<0.05) in TCM groups compared with the model group. The levels of serum cholesterol (TC) and low density lipoprotein(LDL) were declined in TCM group(P<0.05). The contents of TNF-a,IL-1βwere decreased (P<0.05). Compared with the model group and the control group, the blood creatinine in the high-dose group of DZP was decreased significantly (P<0.05). Conclusion Dahuang Zhechong pill suppresses the development of atherosclerosis and reduce kidney damage by modulating blood lipid and anti-inflammation.It is suggested that DZP is beneficial for prevention and treatment on AS-caused renal ischemia and provide some experimental basis for the prevention of atherosclerotic renal artery stenosis (ARAS).