Study On The Dissolution And Transfer Rule During The Fractionation For Glycosides Effective Component Group Of Buyanghaiwu Decoction (BYHWD)

Objection:To clarify the dissolution and transfer rule during the extraction and separation process by detecting the extraction kinetical profile, the solubility peak and dielectric requirement and the column chromatography behavior of macroreticular Resin for glycosides effective component groups of BYHWD,and supply experimental foundation for the orientation and quantification fractionation of the effective component group of the Chinese Medicine Compound Prescription.Method:1.Methods for the content determination and the fingerprint of glycosides effective component group was establishen, which is the differential anthrone-glucose phenylosazone colorimetric method(DAGC);2.By the method of content determination of the glycosides effective component group of BYHWD and the established kinetics model,the kinetical profile deviations and the extraction kinetical curve of glycosides effective component group of BYHWD were carried out; 3.According to the theory of dielectric constants the Solubility peak and dielectric requirement of glycosides effective component group of BYHWD were detected, then the optimal solvent for fractionation of glycosides efficient components was obtained;4.By the column chromatography theory, the optimal velocity and the process parameters for the column chromatography separation behavior of the glycosides effective component group were determined using the optimal mobile phase,and getted the relationship among the quantity of glycosides effective component group of BYHWD, of the stationary phase and the solubility parameter of mobile phaseδm.Result:1.Firstly build the content determination method for glycosides component of the Chinese Medicine Compound Prescription;2.The extraction kinetical profiles for the the glycosides effective component group were fitted three compartment model (R>0.94); The main kinetical parameters tmax,cmax,P,D of the glycosides effective component group of BYHWD were respectively 128.9min,38.17%, 38.16%,24.45%;3.The dielectric requirement of glycosides effective component group of BYHWD was 51.6, and the optimal solvent for fractionation of glycosides efficient components was 50% ethanol solution;4.the optimal velocity for the column chromatography separation behavior of the glycosides effective component group was 2.02 cm/min, and the main process parametersCm Cs K, k were 28.38 mg·mL-1,95.38 mg·mL-1,3.360,8.213 respectively; when the volume of resin was 30 mL, the retention volume of glycosides effective component group of BYHWD was 80 mL with the best column efficiency,and the theory Plate Count and the plate height respectively were 0.3662,40.96.Conclution:It was been validated that the DAGC method was a useful method for the content determination of the glycosides effective component group of BYHWD;It is suggested that for the effective component group of the Chinese Medicine Compound Prescription, the extraction kinetics model was decribed objectively the extraction kinetical profiles, which was a useful tool to study their solubility rules and extraction technique;as the column, the temperature and the stationary phase are all invariable, the orientation and quantification fractionation process for the glycosides effective component group of BYHWD can be carried out by control the velocity, the sample size, the quantity of stationary phase and the solubility parameter of mobile phase δm.