Cytotoxicity Of Cross-linked Dextran Composite Magnetic Nanoparticles And Their Interaction With Cells

Magnetic nanoparticles have many great advantages and widely used in biomedical area, such in clinical diagnostic, thermotherapy and targeted drug delivery due to their special physical and chemical properties. However, the safety is still an important factor and the problem that effects the application of magnetic nanoparticles in biomedical area. The assessment and toxicological study of magnetic nanoparticles becomes a key point to be researched. Cross-linked dextran composite magnetic nanoparticle is developed in our lab which has great magnetic response and drug slow-release properties. The cell lines HepG2, LO2 and ECV-304 were used as models to evaluate the cytotoxicity of cross-linked dextran composite magnetic nanoparticles and studied the interaction between the cells and nanoparticles. Some significant results were obtained and showed blow.1. MTT assay:The cell line HepG2 was used as the object, the method of MTT assay was modified for assessment of cytotoxicity of the cross-linked dextran composite magnetic nanoparticle, and also studies the effects of nanoparticles on the reduction reaction of MTT.2. The cytotoxicity of cross-linked dextran composite magnetic nanoparticle has been evaluated by cell morphology, modified MTT assay, cell growth rate and flow cytometry. The results shown that, the nanoparticles don't have evident cytotoxicity in the range of the concentration of 50—2000μg/ml.3. The preliminary study was made for interaction between the cell and cross-linked dextran composite magnetic nanoparticle by using MTT assay, confocal microscopy and transmission electron microscope. The results shown that HepG2 cell absorb nanoparticles greatly and uptake the nanoparticles by endocytosis.