| Objective To explore the association between the polymorphism of insulin-like growth factor 1 receptor (IGF-1R) gene at nucleotide G3174A and human longevity in Bama County.Methods IGF-1R were genotyped with PCR-RFLP and direct sequencing technique for 187 healthy long-lived people (aged 90-110, longevity group) and their offspring 116 persons (aged 24-79, offspring), 224 local residents (aged 24-79, control group 1) from Bama county and 187 persons (aged 21-79, control group 2) from general district. We compared the differences of allele, genotype of longevity group with other groups.Results 1. No significance diviation from Hardy-Weinberg Equilibrium (HWE) for the polymorphism in IGF-1R gene for the study population was observed (P>0.05). 2. The IGF-1R GA (45.4%) genotype had highest frequency in long-lived elderly, next was GG(41.2%), and AA(13.4%) was lowest frequency. The distribution of GG genotype in offspring group, control group 1 and control group 2 were 51.7%, 53.1% and 52.9%, respectively, the GA genotype were 39.7%, 37.9% and 38.5%, respectively, and the AA genotype were 8.6%, 8.9% and 8.6%, respectively. The G allele frequency in four groups were 63.9%, 71.6%, 72.1% and 72.2%, respectively, the A allele were 36.1%, 28.4%, 27.9% and 27.8%, respectively.3. Compared the differences of allele, genotype of long-lived elderly with the other groups, respectively, showed that (1) There were no significant differences of genotype and allele frequencies between long-lived elderly and their offspring (P >0.05).(2)The GA, AA and (GA+AA) genotype and A allele were found more frequent in long-lived elderly than those in the local control (P<0.05). The values of OR were 1.545(95%CI:1.020-2.341), 1.932(95%CI:1.004-3.716), 1.619(95%CI: 1.094-2.396) and 1.46(95%CI:1.086-1.961).(3) There were no significant differences between long-lived elderly and the pepole from general district in IGF-1R genotype (P >0.05), but the values of OR from AA and (GA+AA) genotype were higher (2.009, 95%CI:1.003-4.024 and 1. 607, 95%CI:1.068-2.419, respectively). The frequence of A allele was found higher in long-lived elderly than that in general district control (P<0.05), the values of OR was 1.466(95%CI:1.076-1.998).4. The serum IGF-1 level of long-lived elderly was (114.0±31.5) ng/ml. There was no difference of serum IGF-1 level in GG, GA and AA genotypes(P >0.05).Conclusion The human longevity in Bama county might be associated with the polymorphism of IGF-1R (G3174A). The GA and AA genotype and A allele of IGF-1R may be protective factor. There is no correlation between IGF-1R genotype and the serum IGF-1 level. Objective To investigate if the levels of p16 and p21 protein expression in serum from people living in Bama county of Guangxi are associated with age, analyze the levels of p16 and p21 protein expression from pepole living in longevity district and general district, and explore the possible relationship between the longevity and p16 and p21 protein.Methods The levels of p16 and p21 protein expression in serum from 201 healthy individuals living in longevity villages of Bama county and 119 healthy individuals from Nandan county were measured by enzyme-linked immunosorbent assay (ELISA).Results 1. The levels of p16 and p21 protein expression in serum from pepole living in Bama county were significantly lower than those of Nandan county (P<0.01). The levels of p16 and p21 protein of different age categories and genders from pepole living in Bama county were all significantly lower than those of Nandan county (P<0.01).2. The levels of p16 and p21 protein expression increased with age in both the pepole living in longevity district and general district.3. The levels of p16 protein between the genders were not different in both the pepole living in longevity district and general district. The levels of p21 protein in female were higher than that in male from the longevity district, but there was no statistical difference in the pepole from general district.Conclusions 1. The levels of p16 and p21 protein expression are much lower in individuals from Bama county than those from general district, and the levels of p16 and p21 protein expression are increasing with age in both longevity district and general district, suggesting that the longevity phenomenon in Bama county might be associated with lower expression of p16 and p21 protein.2. There is no significant gender difference in the levels of p16 protein expression among people from Bama county and general district. |
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