Examination Of IRF7 MRNA And TLR9 MRNA In Patients With Condyloma Acuminate

Background condyloma acuminate (CA)is a common sexually transmitted diseases caused by human papillomavirus (HPV) infection. The current study shows that cellular immunity function of condyloma acuminate patients,especially are recurrent condyloma acuminate patients are suppressed, and the existence of immune balance disorders. A large number of in vitro and animal studies suggest the natural immune response in the inhibition of HPV replication and prevent the spread plays a major role. Interferon regulatory factor family and Toll-like receptor family are closely associated with cellular immunity.Objective To explore the role of IRF7 and TLR9 in condyloma acuminate pathogenesis and clinical significance by examining the expression of interferon regulatory factor 7 (IRF7) and Toll-like receptor 9 (TLR9) in peripheral blood mononuclear cells with condyloma acuminate patients and normal human.Methods 25 patients with condyloma acuminate and 20 normal human Peripheral blood samples were collected,to isolate peripheral blood mononuclear cells by employing lymphocyte separation medium.Then the expressions of IRF7 and TLR9 in peripheral blood mononuclear cells with condyloma acuminate patients and normal human were examined by RT-PCR.Results The condyloma acuminate peripheral blood mononuclear cells showed higher expression of IRF7 mRNA and TLR9 mRNA when compared to normal peripheral blood mononuclear cells(P<0.001), there was significant difference.Conclusion the expression of IRF7 and TLR9 in the condyloma acuminate patients'peripheral blood was highly increased,it probably was associated with the body cell immune response that caused by HPV infection.