Association Study Of Alcohol Dependence And OPRM1 Gene Polymorphism

Objective:Current study has shown that alcohol dependence syndrome is a complex disorder which is influenced by individual inheritance and environmental factors.In this study, we choose Kinetics of drug effects gene- mu opioid receptor gene (OPRM1)。Through the first exon of the gene and analysis of two introns, we hope to obtain the three candidate SNPs genetic polymorphism data. In order to explore the alcohol dependence syndrome gene diagnosis and treatment provide a scientific basis.Methods:According to the《Diagnostic and Statistic Manuel of Mental DisordersⅣ》(DSM-Ⅳ) diagnostic criteria, we select 118 alcoholic dependent patients of Mental Health Center of Yunnan Province and 218 normal persons as control group in the period during 2006-2010.200ul venous blood samples from each person of the alcoholic dependent group and healthy control group were obtained, DNA was extracted by phenol-chloroform method and polymorphism of the three candidate SNPs was assessed by detecting genotypes and allele frequencies by polymerase chain reaction and restriction fragment length technique (PCR-RFLP) and polymerase chain reaction sequence-specific primers .All data were analyzed by statistical software:SPSS 11.5 and HWSIMResults:There are two alleles(A,G) at gene locus rs1799971 and three genotypes (A/A,A/G,G/G) of OPRM1 gene. In the healthy control group:Genotype A/A is found 74 cases(33.9%); Genotype A/G is found 110 cases (50.5%); Genotype G/G is found 34 cases (15.6%); The frequency of allele A is 59.2% and the frequency of allele G is 40.8%; In the alcohol dependence group:Genotype A/A is found 48 cases(40.7%); Genotype A/G is found 53 cases (44.9%); Genotype G/G is found 17 cases (14.4%); The frequency of allele A is 63.1% and the frequency of allele G is 36.9%. There are two alleles(T,C) at gene locus rs1799971 and three genotypes(T/T,T/C,C/C)。In the healthy control group:Genotype T/T is found 126 cases(57.7%); Genotype T/C is found 88 cases (40.3%); Genotype G/G is found 4 cases (0.02%); The frequency of allele T is 71.4% and the frequency of allele C (28.6%); In the alcohol dependence group:Genotype T/T is found 34 cases(28.8%); Genotype T/C is found 84 cases (71.2%); Genotype G/G is not found; The frequency of allele Tis 64.4% and the frequency of alleleC is 35.6%. There are two alleles(G,A) at gene locus rs4870266 and three genotypes(G/G,G/A,A/A)。In the healthy control group: Genotype G/G is found 121 cases(55.5%); Genotype G/A is found 97 cases (44.5%); Genotype G/G is not found; The frequency of allele G is 77.6% and the frequency of allele A is 22.4%; In the alcohol dependence group:Genotype G/G is found 78 cases(66.1%); Genotype G/A is found 40 cases (33.9%); Genotype G/G is not found; The frequency of allele G is 83.1% and the frequency of allele A is 16.9%.Conclusion: There is no significant association of gene loci rs1799971 and rs4870266 between alcoholic dependent group and control group in Han majority of Yunnan province. However, rs648893 gene polymorphism is related to alcoholic dependence. The frequency of three genotypes at this gene locus has statistical significant difference (P<0.05) in both alcoholic dependent group and control group. The frequency of genotype T/T in control group is higher than alcoholic dependent group, T allele might be a postive factor to alcohol dependence. T allele could decrease the incidence of alcohol dependence.