The Influence Of Yu Ping Feng Powder On Cellular Immune Function Of Herpes Simplex Keratitis In Mice Model

Objective Through establishing infected,latent,recurrent herpes simplex keratitis mice model, to discuss the influence of Yu Ping Feng Powder on cellular immune function of herpes simplex keratitis mice model, and to discuss its mechanism of resisting recurring.Methods One hundred and twenty five Balb/c mice were scratched the cornea, among the total of one hundred mice were inoculated with herpes simples virus type-Ⅰ(HSV-Ⅰ), and established primary infected model. The rest of twenty five mice were not inoculated with HSV-I as normal control group. All HSK infection mice were given 0.1% acyclovir eye drop for two weeks to cure the HSK, after four weeks, the HSK latent infection mice model were established. Then the HSK latent infection mice randomly divided into Yu Ping Feng Powder group,Yu Ping Feng Powder and Acyclovir (ACV) group,Acyclovir group,normal saline(NS) control group, each group was twenty five mice. All groups mice were given reciprocal drugs or normal saline(NS) from stomach for three weeks. The eyes of latently infected and control mock-infected mice were exposed to Ultraviolet-B light (UV-B) to induce the recurrence of HSK. On days post-inoculation 28,49 and days 3,7,14 post-UV-B irradiation, randomly elected five mice from each group to process the flow cytometry to examine the contribution of T lymphocyte subsets and the percentage of the herpes virus entry mediator(HVEM). To discuss the effect of Yu Ping Feng Powder on expression of T lymphocyte subgroup(CD3+,CD4+ CD8+) and HVEM; And to process enzyme-linked immunosorbent assay (ELISA) technique to examine the levels of following serum cytokines:interleukin (IL)-2, IL-4, IL-10 and interferon (IFN)-γ. As investigated above, in order to detect the influence of Yu Ping Feng Powder on cellular immune function of herpes simplex keratitis model mice.Results One hundred mice which inoculated with HSV-Ⅰwere established HSK primary infected and latent infected model successfully. Recurrent HSK were in the form of stromal HSV keratitis principally.Compared with normal control group, the percentages of CD3+,CD4+ cell of HSK latent infection model mice were significantly lower, the percentages of CD8+ cell and HVEM of those were significantly higher and the ratios of CD4+/CD8+ significantly lower. Meanwhile, the levels of cytokines of Thl cells (IFN-γ,IL-2) were signifHicantly lower while T helper 2 (Th2) cytokines (IL-10, IL-4) were significantly higher, (P<0.01).After therapied three weeks of latent infection model mice and on days 3,7,14 post-UV-B irradiation, the percentages of CD3+,CD4+ cell and the ratios of CD4+/CD8+were significantly improved and the percentages of CD8+ cell,HVEM of those were significantly lower in the mice of the Yu Ping Feng Powder group,Yu Ping Feng Powder and ACV group than those in the mice of the ACV group and NS control group; Meanwhile, levels of cytokines of IFN-γ,IL-2 were significantly improved and IL-10, IL-4 were significantly lower in the mice of the Yu Ping Feng Powder group,Yu Ping Feng Powder and ACV group than those in the mice of the ACV group and NS control group, (P<0.01).Conclusion Latent infection and recurrent herpes simplex keratitis model mice exist cellular immune function lower and disorder. Yu Ping Feng Powder can play a regulatory and improving role in cellular mimune function in HSK latent and recurrent model mice, and to enhance resisting on HSK recurring.