Effects Of Fuzhen Therapy On Recovery Of Motor Function And Expression Of Nogo-A After Acute Cerebral Infarction In Rats

ObjectiveNowadays the ischemia cerebral vascular disease has threatened the health of human being. The ischemia vascular disease has the character of high morbidity, high mortality rate and high cripples rate. With the improvement of treatment and health care, its mortality has been significantly declined. However, the sequelae of neurology deficits caused by cerebral infarction lead to a heavy burden to Patient and society. In order to ameliorate the quality of patient's life, the further study will focus on the investigations of prevention and treatment strategies.Disability as a leading consequence of neurological deficits after stroke is due to that poor neurite outgrowth and neurogenesis or increasing neurogenesis but not non-function cannot rebuild normal nervous structure following injury of central nervous system. Recent studies demonstrated that neurite outgrowth and such neurogenesis involve the inhibiting factors Nogo-A. Fuzhen therapy is effective to cure stroke. During that, a lot of experiences are accumulated. But the function mechanism is still in study. The subject focus on the influence of Fuzhen therapy on recovery of neurology deficit and expression of Nogo-A in experimental cerebral infarction rats, which provide scientific evidence for clinical experience.MethodsThe health rats were randomly divided into 3 groups. A group (sham-operated group, n=6); B group (ischemia/reperfusion group, n=8); C group (Fuzhen therapy group, n=8). The middle cerebral artery occlulsion (MCAO)was established by using the endothelin-1 method. Fuzhen therapy group were treated for 6 days, the rats of Fuzhen therapy group and ischemia group were tested using beam walking test (BWT) for evaluating motor function after 6h,12h, Id,3d,5d, 7d following ischemia/reperfusion, and the expression of Nogo-A mRNA in the border zone of cerebral infarction were detected by RT-PCR.ResultsThe motor function of Fuzhen therapy Groups showed no significant difference after cerebral infarction for 3 days (P>0.05), but it improved significantly at 5th and 7th day after cerebral infarction (P<0.05). The motor function of the ischemia/reperfusion group showed no significant difference after cerebral infarction for 7d (P>0.05). Compared with ischemia/reperfusion group, The motor function of Fuzhen therapy Groups improved significantly at 5d and 7d after cerebral infarction (P<0.05). The expression of Nogo-A showed no significant difference among the three groups(P>0.05).Conclusions1. Fuzhen therapy can improve the scores of neurological functions deficts. The recovery of the paralyzed extremities was better in the Fuzhen therapy group.2. Because of the limitations of the study, after acute cerebral infarction, the expression of nogo-A showed no significant difference among the three groups. So more studies of fuzhen therapy should be conducted.