Experimental Study On Effect Of Qiong Yu Oral Liquid On Anti-aging

Objective:From studying Qiong Yu oral liquid's function on the behavioral and physical indicators as well as ROS metabolism of subacute aging mice caused by D-galactose to investigate it's effect on the anti-aging, the objective is to provide theoretical foundation and experimental basis for the further research and development of Qiong Yu oral liquidMethods:72 mice are divided into six groups randomly:the blank group, the aging model group, the positive contrast group and the low, the medium and the high three dose groups of Qiong Yu oral liquid. there are 12 mice in each group, In addition to the blank group, mice in the rest of the groups were injected D-galactose (0.05g/10g) daily subcutaneously to manufactur subacute aging mice, blank group were injected saline the same dose with other groups. At the same time, from the first day of the experiment, mice in the three different Qiong Yu oral liquid groups are given Qiong Yu oral liquid in three different dose(0.025ml/10g,0.05ml/10g, and 0. lml/10g) by using ig method every day, mice in the positive contrast group are given liu wei di huang wan also buy using ig mothed. aging model group and blank group are given the same amount of distilled water per day. the above Steps continuou for six weeks, determine all the indicators, in the end of the experiment.1. Study the influence of Qiong Yu oral liquid on the learning and memory of the mice, after the experiment, open field behavior test and step-down test are used to study weather Qiong Yu oral can improve learning and memory of the mice which is caused buy D-galactose.2.Study the influence of Qiong Yu oral liquid on the mice's free radicals, determining the activity of SOD, GSH-PX, and MDA in the serum of the mice, determining the activity of SOD in the mice's brain, and the activity of LPF in the mice'liver. 3. Study the influence of Qiong Yu oral liquid on the weight of mice's immune organs, take each mouse's thymus and spleen at end of the experiment, to determined their index.Results:1. Medium and high dose of Qiong Yu oral liquid could significantly enhance the ability of learning and memory of the mice; improve the capabilities, of explore the new environment of the mice.(1) In the step-down test, the medium dose of Qiong Yu oral liquid can Significantly prolonged the latent period of the mice (P<0.05), can decrease the frequency of learning and the number of errors (P<0.01), high dose of Qiong Yu oral liquid can also decrease the frequency of learning and the number of errors (P<0.05) of the mice.(2). In the open field behavior test, the low, medium and high dose of Qiong Yu oral liquid all can Significantly increase the number of standing(P<0.01) the medium and high dose can increase the grooming frequency (P<0.05), Medium dose can increase the number of throughing lattice (P<0.05)2. Medium and high dose of Qiong Yu oral liquid can increase the antioxidant capacity of the mice, and can reduce the generation of free radicals of the mice.(1) Medium dose of Qiong Yu oral liquid can increase the activity of SOD, GSH-PX in the serum of the mice (P<0.01), increase the activity of SOD in the brain of the mice (P<0.05), increase the weight of mmune organs of the mice (P<0.01orP<0.05), and can decrease the activity of MDA in the serum of the mice (P<0.05), decrease the activity of LPF in the liver of the mice (P<0.O1)(2)High dose of Qiong Yu oral liquid can increase the activity of SOD (P<0.05), GSH-PX (P<0.01) in the serum of the mice, increase the activity of SOD in the brain of the mice (P<0.05), increase the weight of mmune organs of the mice (P<0.05), and can decrease the activity of MDA in the serum of the mice (P<0.05), decrease the activity of LPF in the liver of the mice (P<0.05)Conclusion:1. Medium dose of Qiong Yu oral liquid can play a role in anti-aging through improving the able of learning and memory of the mice as well as improveing the capabilities. of exploring new environment of the mice.2. High dose of Qiong Yu oral liquid can play a role in anti-aging through improving the activity of aging mice's antioxidant enzyme and reducing the generation of aging mice's free radicals, and increasing the weight of immune organs of the aging mice.3..Medium dose of Qiong Yu oral liquid is the best, it's effect on the anti-aging has some relationship with the dose.