| Objective:1 To observe the effectivity and security of pooled hunan platelet gel on wound healing of New Zealand rabbit2 To compare the therapeutic effect between allogeneic platelet gel and conventional therapy on diabetic refractory woundsMethods:1 Animal experiment1.1 Platelet gel preparationPCs (platelet concentrations) were prepared by the single donar method from the whole blood.After the pathogens assays accoding to government standard, eight single-donar PCs were collected to obtain pooled PC and then the concentration of pooled PC were adjusted to 1000×109/L. Pooled PC and activating agents, containing different concentration of thrombin (1000U/ml,100U/ml,0U/ml) in 10% calcium gluconate, were mixed in proportion of 10:1 by the volume rates. The mixtrue were driped to asepsis gauze to get platelet gel gauze.1.2 Experimental animal groupingA total of 24 New Zealand rabbits, female and male a half,were selected and randomly divided into four groups. Every group contained 6 New Zealand rabbits.Whole-thickness skin defects of 3 cm×3 cm were created in the left and right sides of the back of each New Zealand rabbits. The skin defects were treated with different dressing:the control group was asepsis gauze, three experimental groups were platelet gel gauze dressing prepared according to the methods above. At days 5,10,15,20 after operation, the dressing of every defects were changed and tissue specimen of each group were cutted and investigated by histological observation. The raw surface were measured and the healing rate were calculated.2 Clinical research2.1 Platelet gel preparationPCs (platelet concentrations) were prepared by the single donar method from the whole blood.After the pathogens assays accoding to government standard, six single-donar PCs were collected to obtain pooled PC and then the concentration of pooled PC were adjusted to 1000×109/L. Pooled PC and activating agents were mixed in proportion of 10:1 by the volume rates. In the study, there were two different activating agents. The one was 10% calcium gluconate containing 100U/ml thrombin, which was suitable to treat appearance ulcer, the other was 10% calcium gluconate without thrombin, which was suitable to treat sinus.2.2 Case of a particular diseaseA total of 32 diabetes patients including two types of wound, ulcer (n=20) and sinus (n=12), were respectively divided into control groups and experiment groups. The two control groups were treated with routine method while the two experiment groups were treated with PG. The areas of ulcers and the volume of sinus were observed and measured. The results were analyzed by statistical software.Results:1 Animal experimentAlthough the difference on wound healing rate of the control and experimental groups had no significant (P>0.05), the healing quality of three experimental groups were obviously better than the control group by gross. Histological observation showed that the neogenesis skin of three experimental groups have an advantage to the control group:fibroblasts lined up in order and tight, cells proliferated faster, new vesses appeared earlier and more plentiful.2 Clinical researchThe results showed that either the type of wound was ulcer or sinus, experiment groups have an advantage to control groups. The recovery rate of two experiment groups was 87.5%, while the recovery rate of two control groups was 12.5%. The accreting rate of experiment groups, either the type of wound was ulcer or sinus, was significantly higher than the accreting rate of control groups at the third, sixth and tweflth weeks. Experiment groups had an advantage to control groups on the course of treatment, the interval of dressing change and healing quality.Conclusion:1 Pooled humen platelet gel can promote the wound healing of New-Zealand rabbit and it has potential to be applicate to clinical research.2 The healing effects of allogeneic PG on diabetic refractory wounds were better than routine method, no adverse effects were observed in the treatment process. The use of allogeneic PG may provide a simple, safe, and effective means of treating diabetic refractory wounds. |
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