| Di-(2-ethylhexyl) Phthalate (DEHP) is one of the common pollutants of PAEs which have been widely used as plasticizer production of PVC. Every year it was produced about 260 million pounds DEHP. DEHP is used mainly in the following products, such as building materials, automotive products, clothing and medical equipment. DEHP released into the environment through a variety of different ways, mainly released from the long-term use of consumer goods, in the production and packaging process of integration into the resin, industrial waste and indoor waste, waste of burning. DEHP directly released into the air is considered to be a major way into the environment. Soy isoflavones (SI) is a phytoestrogen, which including two main components of isoflavones, genistein and daidzein, which formed in the body with sugar and be in non-activated state. It will generate activation aglycone which may play a certain biological activity when was ingested by bacteria glucosidase hydrolysis in the intestines. It has reported that SI can reduce the risk of atherosclerosis and cardiovascular disease. It also reduces the risk of diabetes the clinical trials.In the study, it explored the oxidative damage with different concentrations of DEHP in mice. More importantly, it explored whether the antioxidant Soy isoflavones can alleviate DEHP-induced damage and protect the body. We may find a substance which can have a preventive effect.In this experiment, consisting of two elements, one is the liver and testicular damage with different concentrations of DEHP in the mice. Four different concentrations includes the control group A (saline), group B (250 mg/kg/day DEHP), group C (500 mg/kg/day DEHP), and group D (1000 mg/kg/day DEHP). The Kunming mice were treated daily with DEHP by gavage for thirty days. They were weighed to determine the dose every day. At the end of experiment, the mice organs of liver, lung, kidney and testis were weighted and the organ coefficients were calculated. Preparation of liver homogenate was to determine the level of malondialdehyde (MDA) and L-Glutathione (GSH) at different experimental groups and GSH level in whole blood. We can observe the lesion of testes of mice through histological analysis. The second part is the highest concentration of DEHP as a control. The mice were administered DEHP (1000 mg/kg /day) and 40 mg/kg/day SI which as a group F. Also it had a group E (only 40 mg/ kg/day SI). The experimental indicators were same with the first part of the experiment. It showed that coefficient of liver, lung and kidney and DEHP concentrations were in a dose-dependent manner. On the contrary, testis coefficient was negatively correlated with the concentrations of DEHP. When the mice both exposed to high concentration of DEHP and SI, SI can significantly improve the organ enlargement, such as liver and lung, also the testis coefficient was return to normal level. With concentrations of DEHP increasing, liver MDA content increased. When fed with the SI supplement, MDA level returned to normal and was close to control group A. The level of GSH was significantly decreased which was dramatically different from the level of MDA. The histological analysis showed DEHP can cause testicular the cavity surface of seminiferous tubules increased, aspermatogenesis, necrosis and massive sloughing at 1000 mg/kg/day DEHP. The state of the seminiferous tubules in group F has been restored to normal morphology.In short, DEHP as an environmental estrogen can cause organs disease and biochemical changes in mice. It was suggested that DEHP made certain oxidative damage and testicular damage. SI supplementation can significantly attenuate oxidative stress and testicular toxicity which may act as antioxidant and estrogen regulator. The study showed SI may protect the DEHP-induced toxicity. It was a still controversial topic that soybean and its biological active component genistein caused the potential health risks. We should be caution of it. |
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