| Objective:To establish and improve an animal model of mice colon cell cancer liver metastases in BALB/c mice, and provides stable platform for the study of liver metastasis mechanism and biological behaviour. Methods:CT26 murine colorectal cancer cell line selected by subcutaneous transfer made after the 3rd generation cell suspension and the CT26 cell suspension were inoculated on BALB/c mouse spleen, after 5min, removal of the spleen. To observe the situation of tumor, liver metastasis rate and life span, and a preliminary comparison of the two models. Experimental group was passed by the subcutaneous inoculation of the spleen after 3 generations, the control group was CT26 cells were inoculated directly to the spleen. Results:Both groups were cloned animal model of colorectal liver metastases, On the 12th day postoperation, the liver metastatic rate 75% in the experimental group were obviously higher than those 28.6% in the control group (P<0.5); on the 15th and 21st day postoperation, the liver metastatic rate (93.7%,100%) in the experimental group were obviously higher than those (50%,66.7%) in the control group (P<0.5), but the metastatic rate to lung, lymph nodes ware no statistical difference between the two groups (P>0.05). The average survival time for the experimental group is (20.56±3.17)d. The control group is (28.14±4.19) d. Pathology result confirmed that liver metastases consistent with typical features of poorly differentiated aden carcinoma. Conclusion:CT26 cells in the immune function of a complete BALB/c mice, still has a good rate of tumor formation and metastasis, and the cell suspensions passage of generations has a shorter tumor formation time but transfer rate is higher.
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