| Colorectal cancer is one of the most common gastrointestinal cancer in the world. Liver is the most common organ of colorectal cancer metastasis, and ultimately up to50%of colorectal caner patients will be died of liver metastasis. Hematogenous metastasis is the main ways of liver metastases from colorectal cancer due to anatomical location. In the early of20th century, researchers had found that the blood from various branches of the portal vein were not mixed uniformly in the portal vein, but flowed to certain parts of the liver in a dog by injecting dye into the different branches of portal vein. Based on this theory, the tumor cells from the primary cancer site of different parts enter to their respective vein and mixture in the portal vein into the liver, whether the growth and distribution of metastases will be affected and presents a different situation? Further more, most studies reported that the number of metastases, size and distribution were the important factors affected the prognosis of colorectal cancer. However, only a few researcher had studied the distribution of metastatic liver lesions from colorectal cancer of different sites by retrospective analysis, there is still much controversy on the conclusions, and the formation process of metastatic liver lesions from colorectal cancer of different sites is rarely reported due to technical constraints. For these reasons, an establishment of liver metastases model to study the correlation of the primary colorectal carcinoma site and liver metastases seems very important.OBJECTIVE To study the correlation of the primary colorectal carcinoma site and liver metastasis by establishing a liver metastasis animal model from colorectal cancer, and explore the anatomical structures and hemodynamic differences of the portal vein of BALB/c mouse.METHODS In this study, the BALB/c mouses were divided into anterior mesenteric vein injection group, posterior mesenteric vein injection group and portal vein injection group, randomly。 EGFP-labeled CT26colon carcinoma cells were injected into the anterior mesenteric vein, posterior mesenteric vein and portal vein to establish a hematogenous metastasis model. Instantaneously observate the growth of the tumor in vivo by fluorescence imaging systems, collected and analysis EGFP fluorescence signal. Fluorescence appearance time in body, liver metastasis foci growth lag phase, rapid growth period, stable period and vitro fluorescence signal doubling time of IOD value, survival, weight, ascites incidence were compared between the groups. Liver metastasis rate, metastases number of the whole liver, constitute of liver lobe metastases distribution, number of metastases liver left and right liver metastases, depth of liver metastases were compared between the groups. RESULTS (1)There were no significant difference in fluorescence appearance time, liver metastasis foci growth lag phase, rapid growth period, stable period and vitro fluorescence signal doubling time of IOD value, survival time of mice, weight change, ascites incidence and depth of liver metastases.(2)The average liver metastases number of the anterior mesenterica vena and portal vein injection group were higher than that of the posterior mesenterica vena injection group, and the difference was statistically significant (F=4.969,P=0.009).(3)The lobe distribution of liver metastases were significant differences in these three groups.(χ2=18.650, P=0.001). In posterior mesenterica vena injection group, the cases of left liver metastases were significantly more than that in right liver or two leaves (χ2=20.222, P=0.000),the anterior mesenterica vena injection and portal vein injection group have no such tendency (χ2=2.250,3.355; P=0.325,0.187,respectively).(4)The total number of liver metastases in left liver and right liver of these groups were significant difference (χ2=34.939,P=0.000), the total number of left liver lobe metastases were higher than that of right liver lobe metastases in mesenterica posterior vena injection group(3.68:1), the left liver metastases tendency was obvious (χ2=45.931, P=0.000). mesenterica anterior vein injection group and the portal vein injection group did not have this tendency.CONCLUSIONS (1)There are no difference in rate of liver metastases, ascites and survival time between anterior mesenteric vein, posterior mesenteric vein and portal vein group (2)The distribution of liver metastases were difference between anterior mesenteric vein, posterior mesenteric vein and portal vein group. Tumou cell from posterior mesenteric vein are easy to be left liver metastases.。 The average liver metastases number of the anterior mesenterica vena and portal vein injection group were higher than that of the posterior mesenterica vena injection group。... |
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