| Objective:Using immunohistochemical techniques detected the expression of SCIN, CDKL1, CUGBP1 and SLC16A7 in colorectal cancer primary lesion. Furthermore, study the relationship between SCIN, CDKL1, CUGBP1, SLC16A7 with clinicopathological characters and prognosis of colorectal cancer liver metastasis. Method:From 2001 to 2009,300 patients who underwent colorectal cancer surgery in the Department of General Surgery of Zhongshan Hospital Fudan University were assigned to research groups. According to the diagnosis and follow-up, we divided them into three groups of 100 cases in each group, which were colorectal cancer without liver metastasis, synchronous liver metastasis, and metachronous liver metastasis. In each group, we used immunohistochemical techniques to detect the expression of SCIN, CDKL1, CUGBP1 and SLC16A7 both in normal tissues and cancerous tissues of colorectal cancer. Using Wilcoxon test, Kaplan-meier survival estimate, univariate and multivariate Cox proportional hazard regression model analyse the relationship between primary focal pathologic features, liver metastasis, survival prognosis and each gene expression status. Result:In colorectal cancer cancerous tissues, SCIN genes was over-active in synchronous liver metastasis compared with colorectal cancer without liver metastasis, (P=0.021). The difference between metachronous liver metastases with colorectal cancer without liver metastasis (P=0.117) and synchronous liver metastasis (P=0.404) was not statistically significant. SCIN gene expressed differently in general type, infiltration, lymph node metastasis, apical node, nerve attack of colorectal cancerliver metastasis. It was up-regulated in ulcer type, T3-T4, lymph node metastasis positive, apical node positive, nerve attack positive of colorectal cancerliver metastasis. In normal tissues of colorectal cancer, SCIN is over-active in synchronous liver metastasis compared with colorectal cancer without liver metastasis (P=0.014). Also SCIN is up regulated in metachronous liver metastases with colorectal cancer without liver metastasis (P=0.019). The difference between synchronous and metachronous liver metastases is not statistically significant (P=0.931). SCIN in cancer tissues is up regulated with normal tissues in colorectal cancer, (P<0.01). CUGBP1 genes in colorectal cancer cancerous tissues:Although the difference between the three groups is not statistically significant, P is near 0.05. There were no difference between CUGBP1 expression and clinicopathological characters. CUGBP1 genes in colorectal cancer tissues:CUGBP1 is over-active in metachronous liver metastasis compared with colorectal cancer without liver metastasis. The difference between the other groups is not statistically significant. CUGBP1 in colorectal cancer cancerous tissues was up regulated with normal tissues in colorectal cancer, (P<0.01). CDKL1 genes in colorectal cancer cancerous tissues:CDKL1 was over-active in metachronous liver metastasis compared with colorectal cancer without liver metastasis. The difference between the other groups was not statistically significant. CDKL1 expressed differently in histology type and infiltration. It was up-regulated in mucinous adenocarcinoma and T3-T4 colorectal tumor of colorectal cancer liver metastasis. CDKL1 genes in normal tissues of colorectal cancer:The difference between each group was not statistically significant. CDKL1 in cancer cancerous tissues was up regulated with normal tissues in colorectal cancer, (P<0.01). SLC16A7 genes in colorectal cancer tissues:SCIN is down regulated in metachronous liver metastasis compared with colorectal cancer without liver metastasis, (P=0.015) and synchronous liver metastasis (P=0.005). The difference between synchronous liver metastasis and metachronous liver metastasis was not statistically significant. There were no difference between SLC16A7 expression and clinicopathological characters. SLCA6A7 gene in normal tissues of colorectal cancer:The difference between each group was not statistically significant. Univariate analyses shows that the happen of synchronous liver metastasis was associated with age, differentiation, infiltration, lymph node metastasis, apical node, nerve attack while metachronous liver metastasis was associated with infiltration, lymph node metastasis, apical node. Multivariate analyses shows that lymph node metastasis positive, apical node positive, SCIN high expression were independent risk factors of the happen of colorectal cancer synchronous liver metastasis. Infiltration station(T3-T4), lymph node metastasis positive, SLC16A7 high expression were independent risk factors of the happen of colorectal cancer metachronous liver metastasis. Univariate analyses shows that colorectal cancer liver metastases overall survival time was correlated with infiltration depth, lymph node metastasis, apical node, nerve attack, SCIN, and CUGBPl. Multivariate analyses shows that apical node positive, nerve attack positive, SCIN gene high expression were independent risk factors of prognosis in colorectal cancer liver metastasis. Conclusion:1, SCIN genes in colorectal cancer tissues was over-active in synchronous liver metastasis compared with colorectal cancer without liver metastasis. SCIN genes in normal tissues of colorectal cancer was over-active in liver metastasis (synchronous or metachronous). It can be used as a forecast index in colorectal cancer liver metastasis. SCIN gene in cancerous tissues was up regulated compared with normal tissues in colorectal cancer.2, CUGBP1 genes in colorectal cancer tissues:The difference in the three groups was not statistically significant. CUGBP1 genes in colorectal cancer tissues: CUGBP1 was over-active in metachronous liver metastasis compared with colorectal cancer without liver metastasis. CUGBP1 in cancer tissues was up regulated with normal tissues in colorectal cancer.3, CDKL1 was over-active in metachronous liver metastasis. It may be used as a forecast index in colorectal cancer metachronous liver metastasis.4, SLC16A7 gene was up-regulated in both synchronous liver metastasis and metachronous liver metastasis compared with colorectal cancer without liver metastasis. It can be used as a forecast index in colorectal cancer liver metastasis.5, SCIN, CUGBP1, CDKL1 in cancerous tissues were up regulated with normal tissues in colorectal cancer liver metastasis.6, Lymph node metastasis positive, apical node positive, SCIN high expression were independent risk factors of the happen of colorectal cancer synchronous liver metastasis. Infiltration station (T3-T4), lymph node metastasis positive, SLC16A7 high expression were independent risk factors of the happen of colorectal cancer metachronous liver metastasis.7, Apical node positive, nerve attack positive, SCIN gene high expression were independent risk factors of prognosis in colorectal cancer liver metastasis.
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