The Expression Of PDGF BB And PDGFR-β In Glomerular And Juxtaglomerular Apparatus Of Patients With Lupus Nephritis, And The Clinical Index In Lupus Nephritis

ObjectiveTo observe the expression of PDGF-BB and PDGFR-βin renal tissue of 50 patients with lupus nephritis including LN type(Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ) and the relationship of with clinical manifestation, including the clearance of endogenous creatine,Complement3,albumin,SLEDAI. To investigate the expression levels of platelet derived growth factor-BB(PDGF-BB) and PDGFR-βand its level in the glomerular and juxtaglomerular apparatus of patients with lupus Nephritis.Methods:The expression of PDGF and PDGFR in renal biopsy specimens of the patients with LN were detected by immunohistochemical stains. We also investigated the relationship between the expression of PDGF-BB and PDGFR in renal tissue in LN and clinical datus.Result:1.The positive expressions of PDGF-BB and PDGFR in LN was upregulated significantly when compared to normal controls.2.Glomerular and Juxtaglomerular apparatus PDGF-BB and PDGFR expresion were different among different pathological type of LN,and the highest was found in type IV LN. than were the typeⅡ,Ⅲ,ⅤandⅠ.3. The expression of PDGF-BB and PDGFR in proliferated LN was higher than those in the non-proliferated LN. 4.In LN, The expression of PDGF-BB and PDGFR in glomerular and juxtaglomerular apparatus were lower correlation with clearance of endogenous creatinine,albumin,C3 and SLEDAI.5.Compared with other types LN, InⅣLN, The expression of PDGF-BB and PDGFR-P were higher significantly; clearance of endogenous creatinine,complement3 was lower than others.ConclusionsOur reseach indicate that in renal tissue the expression of PDGF-BB and PDGFR-P in glomerular and juxtaglomerular apparatus were higher compared with the normal control. In LN, the expressionof PDGF-BB and PDGFR-P in renal tissue was correlated with the condition of renal function. The higher expression of PDGF-BB and PDGFR-β, the more serious damage of the renal function, and it also correlated with the degree of SLE activity. Especially, the expression of PDGF-BB and PDGFR-P was higher in typeⅣLN than the other types. In conclusion, PDGF-BB and PDGFR-βmay participate in glomerular cells and juxtaglomerular apparatus cells proliferation and inflammatory cells infiltration in LN, and may be on factor in the different pathological type formation of LN. PDGF-BB and PDGFR-βplay a very important role in the progression of the end-staged and fibrosis in LN.