Investigation Of The Correlation Between Protein Adsorption And Cell Behaviors On Chitosan/poly(ε-caprolactone) Blend Films

In this study, chitosan/poly(ε-caprolactone) (PCL) blend films with different mass ratios were prepared by spin coating with chitosan/PCL mixture solution. The surface topography and hydrophilicity of the blend films were examined with Atomic Force Microscopy (AFM) and Drop Shape Analyzer (DSA), respectively. The adsorption of fibronectin (FN) on the blend films surface was detected by Quartz Crystal Microbalance (QCM). The adhesion and cell morphology of mouse fibroblast cells (3T3) on the films were also evaluated. Metabolic activity of 3T3 cells cultured on the films was assessed by the MTT assay. The results showed that the topography and hydrophilicity of the blend films were largely related to the mass ratio of chitosan and PCL. The amount of adsorbed FN and 3T3 on the blend films decreased with the increase in chitosan content. In contrast, adhesion and cytoskeleton reorganization of 3T3 cells on the films with lower ratio of chitosan appeared to be largely inhibited.