An NMR-based Metabonomics Research On Disease Of PIH

PIH (Pregancy Induced Hypertension), a common syndrome in pregnant women, severely threatens the life of mother and infant, becoming the main cause of maternal and perinatal mortality. Based on the severity of the disease, PIH is clinically categorized as:gestational hypertension, mild-preeclampsia, severe-preeclampsia and eclampsia. In order to control disease, women with severe-PIH usually receive treatment by taking medicines like Magnesium sulfate. Currently, the exact pathogenic mechanism of PIH is not clear, but the most popular saying is endothelial dysfunction. In this hypothesis, lipid pre-oxidation is a distinct metabolic disturbance.Based on the nuclear magnetic resonance (NMR)-metabonomics approach, This paper explored and analyzed the disease of PIH(severe-preeclampsia). We aim to:1. Monitor the characteristic metabolic reaction in women with PIH; 2.Find the metabolite biomarker which differences women with and without PIH; 3.Supply the data for patheogenesis of PIH.In this research, We collected samples of serum (venous blood,umbilical cord blood)and placenta from normal pregnant women, women with PIH and women with PIH after treatment. It was designed as follows:1.The metabolic profiles of three groups of pregnant women were investigated by NMR.ID-solution NMR and HR-MAS NMR were applied in detecting serum and placenta, respectively; 2. The obtained NMR data were processed by PCA for multivariate statistic analysis;3.The results from NMR experiments and PCA was integrated and explained by reasonable conclusion.Through this research, we find that women with PIH exhibit abnormal metabolite characteristics both in serum and placenta when compared with normal pregnant women and women with PIH after treatment:In venous blood, lipid level is significantly lowered, choline and glycerol are elevated:In placenta, phosphocholine and glycerophosphocholine also increased visibly. But the difference between placenta from three groups of women is not significant due to the limited number of samples. Thus, it is concluded that lipid,choline (both free and in lipid),glycerol in serum can be treated as the biomarkers of PIH disease. However, large number of samples and further researches are needed in this project to reach a high degree of precision and duplicability.