The Expression And Significance Of Autophagy Related Genes Of Beclinl And MAPLC3 In Bone Marrow Mononuclear Cells Isolated From Acute Leukemia Patients

Objective The expression of autophagy-related genes Beclinl and microtubule-associated protein 1 light chain 3 (MAPLC3) in bone marrow mononuclear cells (BMMNC) isolated from acute leukemia (AL) patients was detected and its significance discussed.Methods Transmission electron microscopy (TEM) and RT-PCR were used to detect autophagy activity and Beclinl, MAPLC3 mRNA expression level in BMMNC isolated from 27 AL patients in de novo, refractory or relapse and completely remission (CR) stages and 31 normal persons.Results The results showed that autophagic activity and the Beclinl, MAPLC3 mRNA expression level in BMMNC from de novo AL patients were 80%,0.68±0.18, 0.24±0.06 respectively; those in BMMNC from refractory or relapse patients were 100%,0.79±0.09,0.30±0.07 respectively; those in BMMNC from CR patients were 40%,0.52±0.15.0.16±0.04 respectively; while those in BMMNC from normal control were 20%,0.57±0.13,0.16±0.05 respectively.The autophagic activity and the Beclinl, MAPLC3 mRNA expression level in de novo and refractory or relapse patients were higher than those in normal control with statistical significance (p<0.05), while the comparison between CR patients and normal control showed no statistical difference(p>0.05).Conclusion It is concluded that autophagic activity and the Beclinl, MAPLC3 mRNA expression level in de novo and refractory or relapse patients were higher than those in normal control and the up-regulation in refractory or relapse patients was especially signific(?).This may be related to the (?)sogenesis.development and resistance of AL.