Establishment And Application Research Of The Analysis Method Of Plant-sterol From Chinese Herbal Medicine Which Were Commonly Used In Functional Food

Objective: To establish the standard analysis method of plant sterol by GC technology, and quantitatively determining the distribution and content of plant sterol in about 150 kinds of plants which were commonly used in functional food. The correlation of phytosterol and other nutrients or functional components content in raw materials which were commonly used in functional food has been studied. To provide a direction for raw materials choice.Methods: In establishing the analyzing methods of plant sterol, validation the methods including accuracy, precision, repeatability and calculating the minimum detection limit. According to documents released by the Ministry of Health, about 150 kinds of plants were selected which were commonly used in health food. The content of phytosterol were detected, the proportion, distribution and form of plant sterols in different types of herbal plants were studied. The content of macronutrients(protein and fat),minerals(calcium,iron,magnesium,sodium,potassium) and three common functional components——flavonoids, saponins, alkaloids has been detected by the method in our laboratory; the statistical software SPSS17.0 has been used to describe the distribution of the content of macronutrients,minerals,and three common functional components; and the correlation of phytosterol and other nutrients or functional components has been studied.Results: Each plant sterols were completely separated with few interferential peaks by GC methods. The rate recoveries of campesterol, stigmasterol,β-sitosterol, and stiostanol were respective 88%,90%,95%,92%. In addition, the monomer plant sterols RSD are less than 6%, standard curves were more than 0.99, the limits of plant sterol were (campesterol) 0.2 mg/L,(stigmasterol)0.2 mg/L,(β-sitosterol)0.3 mg/L,(stiostanol) 0.1mg/L . The contents ofβ-sitosterol, campesterol, stigmasterol,β-sitostanol were analyzed by GC methods and the percent of each ingredient were calculated. Phytosterols were detected in all samples ,and different levels in different samples, from 4.0mg/100g to 373.2mg/100g. The samples which were from the plant"flowers"phytosterol average content were highest, at 137.8mg/100g,"seeds"with higher phytosterol, about 97.2 mg/100g. In most samples,β-sitosterol is the largest part of total phytosterol. The average content of"roots"was the same as"others", and its about 61.0mg/100g, the average content of all samples was the same as"leaves"at 75.5 mg/100g, the lowest content of phytosterol was 52.5 mg/100g.β-sitosterol was the most phytosterol in all samples.Nutrients and functional components were not entirely of the normal distribution, so the relationship of phytosterol and other nutrients or functional components used by"spearman correlation analysis", The results showed that: the content of phytosterol and protein ,fat, ash, calcium, magnesium ,total flavonoids content have some correlation, correlation coefficients were 0.295,0.203(P<0.05),0.290,0.226,0.347,0.430, (P<0.01);showed a negative correlation with water, correlation coefficients were -0.182 (P<0.05);the phytosterol content was almost no correlation with iron,sodium,potassium,saponins and alkaloids.Conclusion: The analytical method had high accuracy, precision and repeatability. The choice of GC conditions, making the plant sterols can be well separated, high sensitivity and time was short, easy to implement in other laboratories. The content and distribution of plant sterols of raw materials which were commonly used in Health food and Functional food enriched the database of functional components and conducive to the development of Chinese herbal medicine. There are great significances identifying the relationship between phytosterol and other nutrients or functional components for raw materials choice...