The Function Studies On Fusion Protein Of Transdermal Delivery Peptide TD1 And Epidermal Growth Factor

Transdermal delivery of drugs is with advantages of convenience, sustainalbility and controllability compared with traditional administration including the oral administration and hypodermical injection.It provides a new administration method for desease therapy via the research and application of trasdermal delivery in recent years.TD1 is a short peptide with 11 aa: ACSSSPSKHCG.It has been reported to have the potential ability to facilitate transdermal delivery of macromolecules such as insulin through intact skin to reach systemic circulation to therapy desease by coad-administration.Here we constructed TD1-EGF plasmid and purified the fusion protein through molecular biology methods.We found that the TD1-EGF has the similar cell bio-activity compared with the general EGF such as promoting cell growth,migration and stimulating the phosphorylation of ERK1/2.Meanwile, TD1 can promote EGF to penetrate intact skin more easily.The function of TD1-EGF was time and dose dependent,and need normal body temperature..It may be the result of the interaction between TD1 and skin but not protein.Our research of TD1-EGF in cell bio-activity and transdermal delivery ability provides the rationale of TD1-EGF's application in transdermal drug delivery,wound repair and cosmetic.