Study On FMRI Bran Map In Healthy Adult Undergoing Needing At Zusanli (ST36)

ObjectiveTo observe the characteristic of the fMRI brain map in healthy adult undergoing thehand-motion (clenching and relaxing the fist) and needling at the acupuncture point Zusanli(ST36), for exploring the essence of the fMRI brain map in patients suffering from motordysfunction by cerebrovascular accidents, exploring the essence of the central modulatingmechanism of treatment by acupuncture,and providing imaging evidence to support thetreatment of dyskinesia by acupuncture at Zu San Li (ST36).Materials and MethodThe twelve healthy volunteers were all students from the medical college, six males and sixfemales, all right-handed, aged from 22 to 27 years (average 25.2±1.44 years). None of thesubjects had nervous system disease or a psychiatric history. They did not take any sedatives orreceive acupuncture treatment within three days and two weeks before the trial, respectively. Every subject completed the following tasks: (1) left fist clenching; (2) acupuncture at theleft Zu San Li (ST36)ï¼›(3) right fist clenching; (4) acupuncture at the right Zu San Li (ST36).The data from the brain functional imaging were analyzed with the internationally usedanalysis of functional neuroimages (AFNI) software (edition: 2008₀2₀1₁144). After thereconstruction of the images, the data from the three tasks were aligned based on the third TRthrough time alignment and manual rectification followed by spatial smoothing (FWHM=4 mm).The F values (6, 1121) obtained in the deconvolution analysis from the standardization of thesignal change rate was verified using a t-test. The fMRI imaging was recollected under theTalairach coordinate with a size of 3 mm X 3 mm X 3 mm. The activated area in the functionalimaging was obtained after averaging the results of 12 subjects, and the regions with a signalgreater than three pixels were defined as the activated regions (P<0.005).ResulResult(1)The brain functional regions activated by left fist clenching included the right precentralgyrus, left culmen, right superior temporal gyrus, right culmen, right hippocampus gyrus, rightsuperior temporal gyrus, right medial frontal gyrus, left precuneus, right superior parietal lobule,right middle frontal gyrus and left superior frontal gyrus.(2)The brain functional regions activated by acupuncture at the left Zu San Li (ST36)included the right superior frontal gyrus, right precentral gyrus, left precentral gyrus, rightmedial frontal gyrus, left postcentral gyrus and right postcentral gyrus.(3)The brain functional regions activated by right fist clenching included left precentralgyrus ,left postcentral gyrus,right culmen ,left precentral gyrus, right medial frontal gyrus. (4)The brain functional regions activated by acupuncture at the right Zu San Li (ST36)included left nodule ,left superior temporal gyrus ,right insula , left precentral gyrus ,leftuncus ,right brodmann area 13 , left cingulate gyrus .(5)The may different brain functional regions between activated by left fist clenching andactivated by acupuncture at the left Zu San Li (ST36) included right middle temporal gyrus andleft posterior cingulate .(6)The may different brain functional regions between activated by right fist clenching andactivated by acupuncture at the right Zu San Li (ST36) included right fusiform gyrus .ConclusiConclusion1. Hand motion has its own relative specificity brain region was activated, which issignificant in contralateral precentral gyrus, and dominant in cross function;2. The brain movement-related area was extensively activated by acupuncture at the Zu SanLi (ST36), providing imaging evidence to support the treatment of dyskinesia by acupuncture atZu San Li (ST36).3. The bilateral brain movement-related area was extensively activated by acupuncture atleft Zu San Li (ST36), while only the opposite hemishere movement-related area was activatedby acupuncture at the right Zu San Li (ST36).This provides the basis for reasonable clinicalchoice of acupoints.