The Immunocyte Subsets And Clinical Significance In The Peripheral Blood Of Patients With Immune Thrombocytopenic Purpura

Objective To explore the clinical significance of immunocyte subsets before and after immunosuppressive therapy in the peripheral blood of patients with immune thrombocytopenic purpura (ITP).Method The percentages of immunocyte subsets in the peripheral blood of 35 patients with ITP and 20 healthy controls were detected by flow cytometry, including CD₃⁺,CD₄⁺,CD₈⁺,CD₄⁺/CD₈⁺,CD₅6⁺,CD₁9⁺. All the data were analyzed by statistics software SPSS17.0. Normal distribution measurement data were shown as means±standard deviation ( x±s ). Independent-Sample T Test was applied when we compared the Patients before treatment to control group and Patients after treatment to control group. Paired-sample T test was used when we compared Patients before treatment and Patients after treatment. The inspection standard was alpha = 0.05.Results The percentage of CD₃⁺ T lymphocyte (61.58±6.45)%, CD₄⁺ T lymphocyte (28.38±4.89)% and the ratio of CD₄⁺ and CD₈⁺ T lymphocyte (0.99±0.22) in patients with ITP were lower than those in healthy controls[(67.85±4.68)%,(38.00±3.37)%,(1.54±0.13)]. After immunosuppressive therapy, the percentage of CD₃⁺ T lymphocyte (69.41±5.03)%, CD₄⁺ T lymphocyte (38.17±3.18)% and the ratio of CD₄⁺ and CD₈⁺ T lymphocyte (1.60±0.15) recovered to the control levels. The percentage of CD₈⁺ T lymphocyte (29.20±4.50)% and CD₁9⁺ B lymphocyte (17.74±4.14)% were higher than those in healthy controls [(24.82±2.93)%,(12.09±3.51)%]. After the treatment of immunosuppressive therapy, the percentage of CD₈⁺ T lymphocyte (24.06±3.02)% and CD₁9⁺ B lymphocyte (10.9±3.55)% recovered to the control levels. There were no significant difference of the percentage of CD₅6⁺ lymphocyte among ITP patients (15.80±2.85)%, ITP patients after immunosuppressive therapy (15.16±2.77)% and healthy controls (16.36±2.75)%.Conclusions The present study suggests that there are aberrant immunocyte subsets in the patients with immune thrombocytopenic purpura: the percentages of CD₃⁺T lymphocyte, CD₄⁺ T lymphocyte and the ratio of CD₄⁺ and CD₈⁺ T lymphocyte were decreased; the percentages of CD₈⁺ T lymphocyte and CD₁9⁺ are increased. And the present study suggests that the aberrant immunocyte subsets is involved in the pathogenesis of ITP. Atfter treatment, the aberrant immunocyte subsets in the patients with immune thrombocytopenic purpura are recovery as the platelet counts are above 50 x 109/L. And detection of immunocyte subsets might be helpful for the diagnosis and determination of therapeutic outcome of ITP.