| Objective To observe the therapeutic efficacies of combination of high doseγ-amino butyric acid (GABA), Sodium dimercaptopropane sulfonate (Na-DMPS) and vitamin B6 on liver, brain and heart of rats with acute tetramine intoxication, and compare their efficacies with either GABA or Na-DMPS treatment alone.Methods Thirty rats were randomized into control group (n=6), (only) tetramine treated (alone) group (n=6), GABA group (n=6), Na-DMPS group (n=6) and combination group (GABA+Na-DMPS+VitB6, GNDV,n=6). Blood, liver, brain and heart samples were collected instantly 2 hours after different drugs (GNDV, GABA or NaDMPS) were administrated in rats with acute tetramine intoxication. Serum Alanine aminotransferase (ALT), aspartate aminotransferase (AST), Neuron-specific enolase (NSE) and S-100βprotein, creatine kinase (CK), and creatine kinase isoenzyme (CK-MB) were measured, and pathological changes of the liver, brain and heart were observed.Result:Poisoned symptoms were apparently relieved and the latent period for convulsion/muscular twitch were obviously delayed in poisoned rats administrated with GABA, Na-DMPS or GNDV. Furthermore, combination group showed longer delayed latent period than either GABA or NA-DMPS group. Teatment with GABA, Na-DMPS or GNDV significantly lowered the level of serum NSE,S-100β,ALT, AST, CK and CK-MB in rats with tetramine intoxication. Similarly, combination group had lower serum enzyme levels than either GABA or NA-DMPS group. No significance in serum enzyme changes was observed between GABA and NA-DMPS group. Moreover rats with combination treatment had the slightest pathologic damages in liver, brain and heart (combination>GABA or NA-DMPS)Conclusion:The combination of high doseγ-amino butyric acid, sodium dimercaptopropane sulfonate and vitaminB6 is more effective in treating acute tetramine intoxication. |
PDF Full Text Request