Expression Of HIF 1α, VEGF, CD105 And Their Relationship With Angiogenesis In Osteosarcoma

Objective To investigate the hypoxia-inducible factor-la (HIF-1α), vascular endothelial growth factor (VEGF) and CD 105 expression in osteosarcoma and their relationship with angiogenesis.Methods Immunohistochemical method (SP) was applied to detected the expressions of HIF-1α, VEGF and CD 105 were detected in 36 cases of human osteosarcoma specimen and 10 cases of osteochondroma. We counted microvessel density(MVD) calculated CD105,then used SPSS 18.0 statistics software for analysis.Results 1)HIF-1αprotein expression rate was 41.7% in osteosarcoma, the expression of HIF-1αin osteochondroma was not detected.2) VEGF protein expression rate was 63.9% in Osteosarcoma, the expression of VEGF in osteochondroma was not detected.3)The MVD value of osteosarcoma were significantly higher than that of osteochondroma(P<0.05).4) the expression of osteosarcoma HIF-1αprotein inⅢperiod (metastasis group) was significantly higher than that inⅠ,Ⅱperiod (without metastasis) with statistically significant difference (P<0.05).5) the expression of osteosarcoma VEGF protein inⅢperiod (metastasis group) was significantly higher than that inⅠ,Ⅱperiod (without metastasis) with statistically significant difference (P<0.05).6) The MVD value of osteosarcoma in III period was significantly higher than that in II period(P<0.05).The MVD value of osteosarcoma inⅡperiod was significantly higher than that in I period (P<0.05).7) The expression of HIF-la protein and VEGF protein in osteosarcoma was positively correlated(rs=0.737, P<0.01). The expression of HIF-1αprotein and MVD value in osteosarcoma was positively correlated(rs =0.556, P<0.01). The expression of HIF-la protein and MVD value in osteosarcoma was positively correlated(rs=0.778, P<0.01).Conclusions(1)The proteins of HIF-1αand VEGF had a high expression in osteosarcoma, the expression of HIF-1α, VEGF in Osteosarcoma with metastasis were higher than those without metastasis, which might be involved into the infiltration and metastasis of osteosarcoma.(2)HIF-la is not expressed in osteochondroma but highly expressed in osteosarcoma, it may be a a new marker to judge the potential of benign and malignant bone tumor.(3)The expression of HIF-1αprotein was positively correlated with that of VEGF protein, the expression of HIF-la protein was positively correlated with MVD, the expression of VEGF protein was positively correlated with MVD, Which verificating HIF-la played an important role in the building of osteosarcomas blood vesse] by depending on the expression of VEGF. So it may be a new target for the inhibition of angiogenesis is by restraining the expression of HIF-1α.