The Expression And Clinical Significance Of CD147 And D2-40 In Cervical Squamous Cell Cancer

ObjectiveTo detect the expression of Extracellular Matrix Metalloproteinase Inducer (CD147) and micro-lymphatic vessel density(MLVD) which markered by D2-40 in cervical tissue. To discuss their relationship between CD147, MLVD which markered by D2-40 and clinicopathology parameters in cervical squamous cell carcinoma. To determine the value in prognosticating lymph node metastasis of cervical squamous cell carcinoma.Methods89 cases of cervical tissue were collected, including 11 cases of normal cervical tissue,15 cases of CIN and 63 cases of cervical squamous cell carcinoma. In cervical squamous cell carcinoma, pathological lymph node metastasis was not found in 34 cases and found in 29 cases. Cervical squamous cell carcinoma included 2 cases ofⅠa2 stage,26 cases ofⅠb1 stage,8 cases ofⅠb2 stage,25 cases ofⅡa stage and 2 cases ofⅡb-early stage according to FIGO(2000). Cervical squamous cell carcinoma also included 6 cases of well differentiated tissue,32 cases of moderately differentiated tissue and 25 cases of poorly differentiated tissue according to pathological degree of differentiation, 22 cases of invading superficial myometrium,5 cases of invading middle myometrium and 36 cases of invading deep myometrium according to depth of invasion. With anti-rabbit CD147 monoclonal antibody, anti-mouse D2-40 monoclonal antibody, all samples were underwent immunohistochemistry technique (PV two-step method) by DAB staining. Then we observed their expression of CD147 and MLVD which markered by D2-40 under light microscope and analyzed the data by statistical software SPSS 17.0.Results1. In normal uterine cervical tissue, CIN and cervical squamous cell carcinoma, the positive rates of CD147 were 9.1%(1/11),66.7%(10/15) and 93.7%(59/63), gradually increased, the difference among the three groups was statistically significant (P=0.000). In cervical squamous cell carcinoma, the high expression rates of CD147 in well differentiated tissue, poorly-moderately differentiated tissue were 16.7%(1/6) and 71.9%(41/57), the difference between the two groups was statistically significant(P<0.05). The high expression rates of CD147 in cervical squamous cell carcinoma tissue without lymph node metastasis and with lymph node metastasis were 50.0%(17/34) and 86.2%(25/29), the difference between the two groups was statistically significant(P<0.05). In cervical squamous cell carcinoma, the high expression rates of CD147 had no correlation with age, clinical stage, tumor diameter, depth of invasion and embolus (P>0.05).2. In normal uterine cervical tissue, CIN and cervical squamous cell carcinoma, MLVD markered by D2-40 was 1.55±1.04,7.40±2.87 and 17.33±5.57, the difference among the three groups was statistically significant(P<0.05). In cervical squamous cell carcinoma, MLVD markered by D2-40 in well differentiated tissue and poorly-moderately differentiated tissue were 12.17±2.99 and 17.88±5.52, the difference between the two groups was statistically significant(P<0.05). In cervical squamous cell carcinoma, MLVD markered by D2-40 in invasion of myometrium≤2/3 and >2/3 was 15.56±5.68 and 18.67±5.18, the difference between the two groups was statistically significant (P<0.05). MLVD markered by D2-40 in cervical squamous cell carcinoma without lymph node metastasis and with lymph node metastasis were 14.12±4.90 and 21.10±3.63, the difference between the two groups was statistically significant (P<0.05). In cervical squamous cell carcinoma, MLVD which markered by D2-40 had no correlation with age, clinical stage, tumor diameter and embolus (P>0.05).3. In all cases of cervical tissue, the expression of CD147 and MLVD which markered by D2-40 were correlated positively (rs =0.794,P=0.000). In cervical squamous cell carcinoma, detecting the expression of CD147 and MLVD which markered by D2-40 may prognosticated lymph node metastasis(the area under ROC curve, sensitivity, specificity of CD147 and MLVD which markered by D2-40 were 0.749,58.6%,79.4% and 0.872,75.9%,79.4%). Detecting both of them was more valuable (the area under ROC curve, sensitivity, specificity of their combination were 0.877,82.8% and 82.4%).Conclusion1. The positive rate of CD147 gradually increased in normal uterine cervical tissue, CIN and cervical squamous cell carcinoma. The high expression rates of CD147 in cervical squamous cell carcinoma was significantly higher than normal cervical tissue and CIN, and the high expression rates of CD147 in cervical squamous cell carcinoma was in connection with lymph node metastasis and pathological degree of differentiation. CD147 may be involved in the development and metastasis of cervical squamous cell carcinoma.2. MLVD markered by D2-40 in cervical squamous cell carcinoma was significantly higher than normal cervical tissue and CIN, and in cervical squamous cell carcinoma it was in connection with depth of invasion, pathological degree of differentiation and lymph node metastasis. Micro-lymphatic vessel may be involved in the progress of cervical squamous cell carcinoma.3. In cervical tissue, the expression of CD147 and MLVD which markered by D2-40 were positively correlated. In cervical squamous cell carcinoma, detecting the expression of CD147 and MLVD which markered by D2-40 may prognosticated lymph node metastasis, and detecting both of them was more valuable.