The Specificity Research Of D2-40 And VEGFR-3 To Mark Laryngeal Carcinoma Micro-lymphatic Vessel Density

Objective:To explore the corelation of D2-40 positive micro-lymphatic vessel density (MLVD) and the expression of Vascular endothelial growth factor receptor-3 (VEGFR-3) with metastasis in Laryngeal carcinoma.Analysis the corelation of clinical characters respectively,including age,positions,TNM stage,histological grading.The sensitivity to MLVD between D2-40 and VEGFR-3 were compared.The value of MLVD provid a new way for antitumor therapy,prognosis and the mechalism of metastasis in laryngeal carcinoma.Methods:The expression of D2-40 and VEGRF-3 were detected of the samples from 50 cases of Laryngeal carcinoma,20 cases of polyp of vocal cord and 24 cases of normal laryngeal tissue by Maxvision immunohistochemistry and SABC immunohistochemistry.Then the MLVD marked with D2-40 and VEGFR-3 were examined under optical microscope.And semi-quantitative analysis of the MLVD were examined by HPIAS-1000 image analytical system,Then the correlation between MLVD and the lymph nod metastasis were analyzed,the sensitivity to MLVD between D2-40 and VEGFR-3 were compared by statistical analysis.Results:The expression of D2-40 and VEGFR-3 were different in Laryngeal carcinoma, The MLVD in 50 cases of Laryngeal carcinoma was Significantly higher than in 20 cases of polyp of vocal cord and in 24 cases and normal laryngeal tissue(P＜0.05).The MLVD in 15 cases of lymph node metastasis group were significantly higher than those in 35 cases of non lymph node metastasis group(P＜0.05) and the MLVD in Laryngeal carcinoma was positive corelated to lymph node metastasis.The MLVD in 20 cases type supraglottic,18 cases type glottic and 12 cases of infraglottic laryngeal carcinoma was respectively significantly higher than 8 cases of supraglottic portion,8 cases of infraglottic portion and glottic portion of normal laryngeal tissue (P＜0.05).The MLVD in type supraglottic laryngeal carcinoma was no significant different between the type supraglottic and the tupe glottic laryngeal carcinoma(P＞0.05),but they were higher than type glottic laryngeal carcinoma(P＜0.05).The MLVD in laryngeal carcinoma was no respectively significant different between the age,TNM stage and histological grading(P＞0.05).The statics analysis show the sesitivity to the MLVD of D2-40 were higher than VEGFR-3(P＜0.05).The VEGFR-3 was expressed in cytoplasm and membrane in laryngeal carcinoma tissues,while there was no expression in normal laryngeal tissue.The VEGFR-3 positive rate was 72%(36/50) in 50 cases of laryngeal carcinoma group and 20%(4/20) in 20 cases of polyp of vocal cord group,the positive rate in laryngeal carcinoma group was significant higher than in polyp of vocal cord(P＜0.01).the positive rate in 15 cases of lymph nod metastasis group was significant higher than 35 cases of no lymph nod metastasis(P＜0.05) with the positive rate being 93.33% (14/15) and 62.86%(22/35).The positive rares were respectively 90%(18/20), 44.44%(8/18) and 83.33%(10/12) in 20 cases type supraglottic,18 cases type glottic and 12 cases of infraglottic laryngeal carcinoma.The was no singnificent difference between the type supraglottic and the tupe glottic laryngeal carcinoma(P＞0.05),but they were higher than type glottic laryngeal carcinoma group(P＜0.01).The positive rate in laryngeal carcinoma was no respectively significant different between the age,TNM stage and histological grading(P＞0.05).meanwhile,the VEGFR-3 positive rate was positive correlated to MLVD(P＜0.05).Conclusion:1,the MLVD marked with D2-40 and VEGFR-3 occurred in the tumor stroma tissue predominantly and the tissue near the Laryngeal carcinoma and the was no MLVD in Laryngeal carcinoma nests.The MLVD in Laryngeal carcinoma group was higher than those of polyp of vocal cord group.2,MLVD in Laryngeal carcinoma was positve correlated with cervical lymphatic metastasis and positions,and uncorrelated with age,TNM stage,histological grading.3,The VEGFR-3 positive rate was positive correlated to MLVD in Laryngeal carcinoma group and polyp of vocal cord group.4,D2-40 is a specific immunohischemical marker for lymphatic endothelialioma with higher sensitivity than VEGFR-3.it can specifically mark the MLVD and can not mark the vessel in Laryngeal carcinoma.It can extensively used in marking MLVD in Laryngeal carcinoma.