| The research consist the following three experiments:1 To compare the pathologic changes and iconographic changes of lumbar facet joint osteoarthritisObjective:To compare the pathologic and imaging changes seen in lumbar facet joint osteoarthritis. Methods:A totally of 20 patients (male 8, female 12) undergoing posterior lumbar spinal surgery in our department were evaluated regarding the extent of degenerative arthrosis according to the Weishaup grading criteria. We used toluidine blue staining to observe histological changes in articular cartilage and graded the extent of degenerative cartilage using the classification method proposed by Nicloa. Results:When comparing the imaging changes with thoes seen in histological studits,we obsorved grade 3 in one when imging grade was grade 0; grade 1 degeneration in one case,grade 3 in one and grade 4 in one when imaging grade was grade 1; finally, grades 3 and 4 degeneration corresponded to imaging changes of grades 2 and 3. Conclusion: The pathological changes was consistent with the lesion and necrosis of degenerative cartilage. When iconographic classification was grade 0 and 1, cartilage classification was more severe and was obviously imaging with the imaging greading. But, when imaging grades was grade 2 and 3, the cartilage classification was all grade 3 and 4. They were highly consistent with each other. This phenomenon suggested that CT and MRI have some limitations in the early diagnosis of facet joint osteoarthritis, but they may provide a good reference in the diagnosis of late facet osteoarthritis.2 To initially identify the expression of inflammatory factors in lumbar facet joint osteoarthritis and determine that whether these factors associate with pain.Objective: To initially approach the role of lumbar facet joint derived inflammatory factors in degenerative lumbar spinal canal stenosis; Method: Totally 75 cases of degenerative lumbar spinal canal stenosis(41 cases) and lumbar intervertebral disc herniation(34 cases)undergoing posterior lumbar spinal surgery in our department were evaluated extent of degenerative arthrosis according to the Weishaup grading criteria. The grading of backleg pain, melosalgia and functional impairment were recorded. The excisional lumbar facet joints were collected as species. The content of interleukin-1βand tumor necrosis factor-αin the species were determined by ELISA. Results: there was no TNF-αdetected in both of the two groups. More IL-1βwas detected in degenerative lumbar spinal canal stenosis group than that in lumbar intervertebral disc herniation group. It was demonstrated that the content of IL-1βin the species increased as the degeneration of lumbar facet joint sharpened. In degenerative lumbar spinal canal stenosis group, the grading of backleg pain, melosalgia and functional impairment was apparently higher than when IL-1βwas detected while there was no statistical difference in all the species in lumbar intervertebral disc herniation group. Conclusion: the cartilage of degenerative lumbar spinal canal produced more IL-1β. Lumbar facet joint derived inflammatory factors might be one of the reasons that cause backleg pain and melosalgia and functional impairment in degenerative lumbar spinal canal stenosis patients.3 To evaluate the expression of TNF–α,IL- 1βand NO in rat lumbar facet joint osteoarthritis induced by collagenase.Objective: to initially explore the changes in the expression of TNF–α,IL- 1βand NO in the development of facet joint osteoarthritis. Method: Adult SD rats (48 cases) were randomly divided into two groups: collagenase injection group (24 cases),normal control group (24 cases). Osteoarthritis were induced by intra-articular injection of collagenase. After 1 week, 2 weeks,4 weeks,8weeks 6 rats of each group were killed and L5/6 facet joint were taken out immediately. Dynamic changes in cartilage tissue were continuously observed under inverted microscope.IL-1βand TNF-αin the arthrodial cartilage were detected by ELISA. Nitric oxide synthase were detected by immunohistochemical staining and were quantitatively analyzed by cell image analysis. Results: In the osteoarthritis model induced by collagenase, articular cartilage damage increased with time. Immunohistochemistry of iNOS showed that, in superfacial cartilage there are small amount of straining after 1 week. After 2 weeks, positive staining increased obviously. After 4 weeks, positive staining increased significantly and was observed in the middle and subnatant cartilage. After 8 weeks, more positive strain was observed in the full-thickness cartilage; After 1 week, the staining intensity of iNOS compared with the control group increased significantly. After 2 weeks, 4 weeks and 8 weeks, iNOS staining intensity has remained at a high level; IL-1βand TNF-αin the collagenase injection group were significantly higher than the control group. After 2 weeks, IL-1βreached the peak, then decreased gradually. After 8 weeks, IL-1βwas still at a high level. TNF-αincreased significantly after 1 week and decreased after 2 weeks. After 4 weeks, it decreased significantly. After 8 weeks, there was no significant difference between the two groups. Conclusion: The pathological changes in the osteoarthritis model induced by collagenase injection was similar with that of patients with facet joint degeneration. And degeneration of cartilage gradually increased with the course of disease; Facet joint-derived IL-1βwas positively correlated with the extent of degeneration in the cartilage, but it didn't increase with the aggravation of cartilage degeneration. TNF–αand IL - 1βmaybe act at different stages of inflammation and TNF–αact in early stage. The content of iNOS in degenerative aritcular cartilage increased with time suggesting that NO may play an important role in the course of osteoarthritis. |
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