The Research Of The Relationship Between Helicobacter Pylori And Cholecystolithiasis

Objective:To investigate the relationship of Helicobacter pylori with cholecystolithiasis.Methods:ELISA and immunohistochemistry approach were used to detect Helicobacter pylori from gallstone, bile and gallbladder mucosa in 47 Cholecystolithiasis Patients (experimental group) and 26 patients without gallstone (control group)Results:In Cholecystolithiasis Patients, the positive rate of Helicobacter pylori in gallbladder mucosa, bile were 20.6 and 48.6. The positive rate of Helicobacter pylori in bile was significantly higher than that in gallbladder mucosa, there was significant difference (p<0.05). In patients without gallstone, the positive rate of Helicobacter pylori in gallbladder mucosa and bile were 26.3 and 55.0, the positive rate in bile was significantly higher than that in gallbladder mucosa, there was significant difference (p<0.05). Compared with patients without gallstone, the positive rate of Helicobacter pylori of Cholecystolithiasis patients in gallbladder mucosa and bile was 20.6v.s.26.3 and 48.6v.s.55.0 (p>0.05, respectively). In the positive samples by immunohistochemistry, Helicobacter pylori was found in epithelium surface of gallbladder mucosa and gland cava, and was spiral shaped, short spiral or globular shaped. Helicobacter pylori was staining mainly concentrated in the cells around, and stained brown. Helicobacter pylori was obviously rare compared to rod-shaped bacteria. Some of these bacteria scattered, and some were cluster-like distribution.Conclusion:In this study, gallbladder and bile samples of two groups of patients with gallstone and non-gallbladder stones were used immunohistochemistry and enzyme-linked immunosorbent assay method to detect H. pylori, indicating that both gallbladder and gallbladder bile of patients with gallstone or non-gallstone were in the presence of Helicobacter pylori infection. But the final results show, the positive rate of H. pylori infection in stone group and non-stone group was no significant difference, so that the relationship between Helicobacter pylori infection and the formation of gallstones is not clearly, but it needs further research that whether helicobacter pylori and its metabolic products can indirectly change the kinetics of biliary tract and cholesterol by changing the level of enzymes and hormones through other ways or not, and this kind of changes can promote the process of lithogenesis. The researches involving the relationship beteewn H. pylori and lithogenesis are only limited to the detection of H. pylori DNA and its relative immune proteins.We suspect that the function of those DNAs and proteins in lithogenesis is maybe explained as follow:those DNAs and proteins retrogradely infect the biliary tract through the sphincter muscle of hepatopancreatic ampulla,and the infection may destroy the mucous membrane of gallbladder,and the massive metabolite cause the lithogenesis.lt needs further research to verification the exact roles of H.p and its metabolites in lithogenesis through various pathological changes.