The Research Of The Function Of Vacuum-assisted Closure In The Progress Of The Chronic Wound Healing

Objective: To discuss the mechanism of V.A.C in the healing of chronic wound .Method:1 30 inpatients who have chronic wound (the date of being ill was over 1 month, including pressure sore, burn, diabetes foot and ulcer) were included in this research. They were divided into two groups, 15 patients in each group. Experiment(E) group were treated by V.A.C, and Control(C) group were treated by common dressing change. All the other treatments were similar in both groups .2 A piece of tissue measured 0.3cm×0.3cm was taken in the E group on the first day of treatment as the sample before treatment and it was put into formalin. Another same size sample was taken seven days after the V.A.C treatment was performed which was the sample after treatment.During the treatment, the pressure value was -125mmHg.Samples were taken from patients in C group in the same way on the first day when they were accepted in hospital as the sample of before treatment.Seven days after dress changing, another same size tissue was taken as the after treatment sample.Thirty samples were taken in both groups.3 Thirty examples in both groups were fixed and paraffin embedded.Slides of each sample were stained by HE, TP/PD-ECGF and CD34 immunohistochemical dyed methods. These slides were observed by microscope.MVD and TP count should be statisted. All the data were analysed by SPSS13.0,MVD counts were analysed by T-test after Test forNormality and epual variance,TP/PD-ECGF counts were analysed by Chi-Square test, and the P value less than 0.05 was taken as significant difference. Results:1 HE group: The changes of slides in E group after and before treatment were more siginifcant than in C group.Increasement of the V.A.C group changed more significant than the common group. And the V.A.C group Aizen components in E group after treatment were more than in C group.2 CD34 group: Significant difference of counts of MVD by CD34 signed before and after treatment were found in both groups.Significant difference was found between the two groups .3 TP/PD-ECGF group: Positive reaction of slides stained by TP/PD-ECGF was found mainly in E group after treatment.Less was found in C group. The change of positive reaction of TP/PD-ECGF stained in E group was significant, while there was little change in C group.Conclusion : It can be concluded that the treatment of V.A.C can play a more effective role than common dressing change in stimulating tissue regeneration,improving local circulation and promoting platelets gathering on the chronic wound which lead to the acceleration of the tissue rehabilitation and wound healing when the wound bed is filled with blood.