Plasma Metabonomics Study On The Asthma Diagnosed As Shenxu-tanyu Syndrome In Traditional Chinese Medicine With NMR

Objective To application the metabonomics technology about nuclear magnetic resonance (NMR), Retrieval substance metabolism way ,metabolism state, discover biological marker in asthma diagnosed as Shenxu-tanyu syndrome patients ,by analysing and judging the interrelated specially component about plasma with asthma diagnosed as Shenxu-tanyu syndrome patients. Methods Sodium phosphate buffer was employed to prepare plasma samples which obtained from 39 cases of Shenxu-tanyu syndrome patients with asthma and 40 healthy volunteers. A 1H NMR based metabonomic analysis was performed on these plasma samples. A multi-steps date preprocessing procedure was completed and then Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA) was fulfilled to distinguish the metabolic phenotypes, the influential biomarkers accounting for the metabolic signature perturbation were discovered by Pearson's product moment correlation coefficient and 2D NMR. Results It was found that the metabolic phenotypes in Shenxu-tanyu syndrome with asthma group underwent signification variation , which resulted in the obvious classification between the health and patients groups in the score scattering plot of OPLS-DA model; the results show that in plasma of Shenxu-tanyu syndrome patients with asthma the expression of isoleucine , leucine , valine , lactate , alanine , glycoprotein , citrate , creatine , creatinine , 1-methy histidine , scyllo-inositol see a significant decrease comparing with health group (P<0.05) , whereas , the expression of acetone , acetoacetate , carnitine significant elevated (P<0.05). Conclusion Compared with asthma diagnosed as Shenxu-tanyu syndrome patients,there were significant differences in metablolite and metablism net in healthy, possible by interaction between geneticfacter and environmental facter. The marker metablolite of asthma diagnosed as Shenxu-tanyu syndrome have osculate correlation with pathologic physiology change ,such as airway inflammation ,airway hypersensitivity ,airway remodeling . meanwhile, during asthmatic attack period , the process of energy metabolism have significant changed in the asthma diagnosed as Shenxu-tanyu syndrome patients, particular expression in increasing metabolism fatty acid and branched-chain amino acid, restraining tricarboxylic acid cycle, strengthening glyconeogenesis, reducing compound and metabolism creatine, prediction that asthma diagnosed as Shenxu-tanyu syndrome patient have continued analosis and immunologic function disorder in long time.the continuance and protraction stimulation in airway inflammation is main reason to lead to this condition. It provided the preliminary foundation in recognizing further microcosmic physicala foundation of sthma diagnosed as Shenxu-tanyu syndrome and evaluating the intervene effect of Traditional Chinese Medicine to asthma diagnosed as Shenxu-tanyu syndrome.